Concept paper on SI value assignment of purity - Model for the classification of peptide/protein purity determinations Ralf D. Josephs, Norbert Stoppacher, Steven Westwood, Robert I. Wielgosz, Ming Li, Milena Quaglia, Jeremy Melanson, Gustavo Martos, Desiree Prevoo, Liqing Wu, Sandra Scapin, Merve Öztug Senal, Lingkai Wong, Ji-Seon Jeong, Kelly Wai Yi Chan, Cristian G. Arsene and Sang-Ryoul Park J. Chem. Metrol. (2017), 11:1 ; 1-8 Bureau International des Poids et Mesures (BIPM), Sèvres, France National Institute of Metrology (NIM), Beijing, P. R. China LGC, Teddington, United Kingdom Measurement Science and Standards, National Research Council (NRC), Ottawa, Canada Laboratoire National de Métrologie et d’Essais (LNE), Paris, France National Metrology Institute of South Africa (NMISA), Pretoria, South Africa National Institute of Metrology, Quality and Technology (INMETRO), Duque de Caxias, Brazil National Institute of Metrology (TUBITAK UME), Gebze-Kocaeli, Türkiye Health Science Authority (HSA), Singapore Korea Research Institute of Standards and Science (KRISS), Daejeon, South Korea Government Laboratory (GLHK), Kowloon, Hong Kong SAR China Physikalisch-Technische Bundesanstalt (PTB), Braunschweig, Germany Abstract: Required metrological tools, such as higher order reference measurements procedures, pure substance and matrix certified reference materials, are established for small well defined molecules. Difficulties still remain in the provision of SI traceable standards in the area of larger biomolecules such as peptides/proteins. The provision of Primary Calibration Reference Services has been identified as a core technical competency for National Measurement Institutes (NMIs). A concept has been elaborated by the Focus Group I on peptide/protein purity for the strategic planning of ongoing Key Comparisons of the Protein Analysis Working Group (PAWG) within the Comité Consultatif pour la Quantité de Matière (CCQM). The assignment of the mass fraction content of high purity peptide/protein materials will be the subject of the CCQM-K115/P55.2 comparison series to directly support NMI services and certified reference materials currently being provided by NMIs. Keywords: Peptide; protein; purity; traceability; comparison. © 2017 ACG Publications. All rights reserved. |
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