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Determination of sulfonamides in milk by ID-LC-MS/MS

Isin Aydin Unsal, Murat Tasan, Taner Gokcen and Ahmet C. Goren

Namik Kemal University, Department of Food Engineering, P.O. Box 59030, Merkez Tekirdag, Türkiye

TUBITAK UME, Organic Chemistry Laboratory, PK 54, 41470,Gebze Kocaeli, Türkiye

Bezmialem Vakif University, Faculty of Pharmacy, Department of Analytical Chemistry,34093, Fatih-İstanbul, Türkiye

Abstract: In this study, an ID-LC-MS method was developed and validated for the determination of 14 sulfonamides in milk samples. Recovery results were found to be in the range of 91%-114% for milk samples. The relative measurement uncertainty was between 7.5%- 12.7%. Validated method was performed on milk samples obtained from market and street vendors. The amount of sulfonamides in the analyzed samples was found to be below the legal limits (Sulfamethazine: 6.46±0.76 ng/g and sulfisoxazole: 7.3±0.71 ng/g).

Keywords:Sulfonamides, isotope dilution; LC-MS/MS, method validation; uncertainty estimation. © 2018 ACG Publications. All rights reserved.