2.

Development and Validation of High Performance Thin-Layer Chromatographic Method for Determination of α-Mangostin in Fruit Pericarp of Mangosteen Plant (Garcinia mangostana L.) using Ultraviolet – Visible Detection

Himanshu Misra, Binit K. Dwivedi, Darshana Mehta, Bhupendra K. Mehta, and Dharam C. Jain

School of Studies in Chemistry & Biochemistry, Vikram University, Ujjain – 456 010, Madhya Pradesh, India
Green Technology Department, Ipca Laboratories Limited, Ratlam – 457 002, Madhya Pradesh, India

Abstract: A simple, fast and precise quantitative high performance thin-layer chromatographic method has been developed for quantitative estimation of α-mangostin in fruit pericarp of Garcinia mangostana L. (Hypericaceae). Best solvent for extraction of a-mangostin optimized after screening with five solvents under same conditions using hot solid-liquid extraction through soxhlet apparatus. Methanol and chloroform gave highest and second highest recovery of a-mangostin, respectively. Plates were developed in chloroform-methanol in the ratio of 27-3 (v/v). Post-chromatographic derivatization performed using anisaldehyde-sulphuric acid reagent and scanned at 382 nm in ultraviolet-visible mode. The developed method was found to be linear in the range 1.0 to 5.0 mg spot-1, limits of detection and quantitation were 150 and 450 ng spot-1. The developed method was validated in terms of system suitability, specificity and robustness.

Keywords: Garcinia mangostana L.; Hypericaceae; α–Mangostin; HPTLC determination.