Gas Chromatographic Determination of Quinolizidine Alkaloids in Genista sandrasica and Their Antimicrobial Activity
Nurgün Küçükboyacı, Semiha Özkan and Fatma Tosun
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Türkiye
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Gazi University, 06330 Ankara, Türkiye
Abstract: Alkaloid profile of the aerial parts of Genista sandrasica Hartvig & Strid. (Fabaceae) growing in Turkey was studied by capillary gas chromatography-mass spectrometry (GC-MS). Ten quinolizidine alkaloids were identified in the alkaloid extract of G. sandrasica . The main alkaloids were: sparteine (13.68%), N-acetylcytisine (6.48%), 13-methoxylupanine (13.12%), anagyrine (40.49%) and baptifoline (10.76%). In addition, antibacterial and antifungal activities of the alkaloid extract of G. sandrasica were tested against standard strains of the bacteria (Escherichia coli , Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus) as well as the fungi (Candida albicans and Candida krusei). The alkaloid extract of G. sandrasica showed significant activity against B. subtilis and S. aureus with minimum inhibitory concentrations (MIC) of 31.25 and 62.5 µg/mL.
Keywords: Genista sandrasica ; Fabaceae; alkaloids; GC-MS; antimicrobial activity. |