24.

LC-DAD-UV and LC-ESI-MS-based Analyses, Antioxidant Capacity, and Antimicrobial Activity of a Polar Fraction from Iryanthera ulei Leaves

Freddy A. Bernal, Luis E. Cuca-Suárez, Lydia F. Yamaguchi and Ericsson D. Coy-Barrera

Laboratorio de Productos Naturales Vegetales, Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, AA 14490, Bogotá, Colombia.

Laboratório de Química de Produtos Naturais, Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, SP CP 26077, 05599-970, Brazil.

Laboratorio de Química Bioorgánica, Departamento de Química, Facultad de Ciencias Básicas y Aplicadas, Universidad Militar Nueva Granada, AA 49300, Cajicá, Colombia.

Abstract: LC-DAD-UV and LC-ESI-MS-based analyses were performed in order to chemically characterize a phenol-enriched fraction obtained from Iryanthera ulei leaves-derived ethanol extract. Eight glycosylated flavonoids, two free-flavonoids and two neolignans were detected to be part of its isopropyl acetate-soluble (iPS) fraction. Presence of afzelin 1 was confirmed by isolation. Total Phenolic (TPC) and Total Flavonoid Contents (TFC), Antioxidant Capacity (DPPH, ABTS • + , and FRAP methods), as well as antimicrobial activity against five strains were determined.

Keywords: Myristicaceae; Iryanthera ulei ; LC-UV-DAD; LC-ESI-MS; Flavonoids; Antioxidant; Antimicrobial.