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Sesquiterpene Lactones of Amphoricarpos autariatus ssp. autariatus from Montenegro - Antifungal Leaf - Surface Constituents

Milka Jadranin, Iris Đorđević, Vele Tešević, Vlatka Vajs, Nebojša Menković, Marina Soković, Jasmina Glamočlija and Slobodan Milosavljević

Institute for Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000, Belgrade, Serbia

Faculty for Veterinary Medicine, University of Belgrade, Bulevar oslobodenja 18, 11000 Belgrade, Serbia

Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, PO Box 158, 11001 Belgrade, Serbia

Institute for Medicinal Plant Research, "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia

Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia

Abstract: The composition of leaf cuticular neutral lipids of Amphoricarpos autariatus ssp. autariatus collected at canyon of river Tara (North Montenegro) was investigated by GC/MS (nonpolar fraction), LC-ESI TOF MS and 1H NMR spectroscopy (more polar fraction). The nonpolar fraction (ca. 15% of the whole surface extract) contained C 27 - 33 n-alkanes, those with odd-number of carbons predominating. The LC-ESI MS and 1H NMR of the more polar fraction revealed 13 sesquiterpene lactones, constituting ca. 97.5% of the lactone mixture, identified as the known guaianolides, so-called amphoricarpolides, found previously in the aerial parts of the genus. The lactone fraction exhibited considerable in vitro effect against eight fungi, i.e. Aspergillus ochraceus , A. niger, A. versicolor , Penicillium funiculosum, P. ochrochloron, Trichoderma viride, Fusarium verticillioides and Fulvia fulvum.

Keywords: Amphoricarpos autariatus ssp. autariatus; Asteraceae; Leaf-surface sesquiterpene lactones; Amphoricarpolides; Antifungal activity.