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Phenolic Compounds of Pinus brutia Ten.: Chemical Investigation and Quantitative Analysis Using an Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry with Electrospray Ionization Source

İbrahim Kıvrak, Şeyda Kıvrak, Mansur Harmandar and Yunus Çetintaş

Muğla Sıtkı Koçman University, Research Laboratory Center, MGL Food Analysis Laboratory, 48000 Kotekli, Mugla, Türkiye

Department of Chemistry, Faculty of Science, Muğla Sıtkı Koçman University, 48000 Kotekli, Mugla, Türkiye

Abstract: In this study, phenolic content of Pinus brutia ’s bark was examined using an ultra-performance liquid chromatography tandem mass spectrometry with electrospray ionization source (UPLC-ESI-MS/MS) working in multiple reaction monitoring mode. U ltrasonic extraction method with 50% ethanol solution was used for the extraction of bark. The bark of Pinus brutia consisted of 15 compounds: gallic acid, gentisic acid, protocatechuic acid, 4-hydroxy benzoic acid, catechin hydrate, vanillic acid, caffeic acid, vanillin, p-coumaric acid, ferulic acid, myricetin, resveratrol, luteolin, naringenin, kaempferol. Major compound detected was catechin hydrate (28.305 mg 100 g -1 extract). The phenolic compounds of Pinus brutia extract and pycnogenol were compared, and it is shown that both of them consisted of considerable amount of phenolic compounds.

Keywords: Pinus brutia ;Pycnogenol; Phenolics; UPLC-ESI-MS/MS