5.

Gastroprotective Effect of the Ethanolic Extract and Fractions obtained from Syngonanthus bisulcatus Rul.

Leônia Maria Batista, Ana Beatriz Albino de Almeida, Gedson Rodrigues de Morais Lima, Heloina de Sousa Falcão, Anderson Luiz Ferreira, Luciana de Pietro Magri, Roberta Gomes Coelho, Tamara Regina Calvo, Wagner Vilegas, Alba Regina Monteiro Souza Brito

Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brasil.

Departamento de Fisiologia e Biofísica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Cx. Postal 6109, CEP. 13083-970, Campinas, SP, Brasil.

Departamento de Química Orgânica, Instituto de Química, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Araraquara, SP, Brasil.

Abstract: Syngonanthus bisulcatus Rul., popularly known in Brazil as “sempre-vivas chapadeira”, is a plant of the family Eriocaulaceae, it is found in the states of Minas Gerais and Bahia. In this work, the ethanolic extract (EtOHE), flavonoid-rich (FRF), and flavonoid-deficient (FDF) fractions obtained from scapes of S. bisulcatus were investigated for gastroprotection in both rats and mice. The activity was evaluated in models for induced gastric ulcer (absolute ethanol, stress, non-steroidal anti-inflammatory drugs, and pylorus ligation). The participation of mucus and prostaglandin E 2 were also investigated. Sb-EtOHE (50, 100, and 250 mg/kg, p.o.), Sb-FRF (100 mg/kg, p.o.), and Sb-FDF (100 mg/kg, p.o.) significantly reduced gastric injuries in all models. Sb- FRF altered gastric juice parameters after pylorus ligation. Sb-FRF and Sb-FDF (100 mg/kg each, p.o.) significantly increased the amount of adherent mucus in the gastric mucosa. Sb-FRF maintained the mucosal levels of prostaglandin after the administration of indomethacin. The results indicate that Sb-EtOHE, Sb-FRF and Sb-FDF have significant gastroprotective activity. The observed gastroprotective effects of S.bisulcatus probably involve the participation of both mucus and prostaglandins, integral parts of the gastrointestinal mucosa’s cytoprotective mechanisms against aggressive factors.

Keywords: M edicinal plants ; Eriocaulaceae; Syngonanthus bisulcatus; Gastric ulcer; Gastroprotective activity .