13.

Two New Monoterpenes from Tithonia diversifolia and Their Anti-Hyperglycemic Activity

Xia Li, Guanghui Huang, Guijun Zhao, Wansheng Chen, Junli Li and Lianna Sun

School of Pharmacy, Second Military Medical University, Guohe Road 325#, Shanghai 200433, P.R China;

Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, Fujian, PR China

Department of Pharmacy, Chanzheng Hosbital, Second Military Medicinal University, Fengyang Road 415, Shanghai 200003, P.R. China

Abstract: T wo new monoterpenes : ( (1S,2R,3R,5S)-2-hydroxymethyl-6,6-dimethylbicyclo[ 3.1.1]heptane-2,3-diol (1) and (3R)-6,6-dimethyl-4-methylenebicyclo[3.1.1]heptane-1,3-diol-3-O- b -D-glucopyranoside (2), along with three known compounds, namely, sobrerol (3), (1R,2S,5S)-2,8-p-menth-diol (4) and (lR,5S)-10-hydroxyverbenon (5), were isolated from aerial part of Tithonia diversifolia. Their structures were determined on the basis of spectroscopic analyses (IR, HR - ESI-MS/MS, 1D/2D NMR). Under the concentration of 10 m g/mL, compounds 1 and 3 significantly increased glucose uptake in 3T3-L1 adipocytes without significant toxic effects in vitro.

Keywords: Tithonia diversifolia ; Monoterpene; 3T3-L1 adipocytes; Anti-hyperglycemic activity.