Untitled Document

Records of Natural Products

Year: 2014 Volume: 8 Issue: 2

ORIGINAL ARTICLE

Daphne oleoides Schreber ssp. oleoides Exhibits Potent Wound Healing Effect: Isolation of the Active Components and Elucidation of the Activity Mechanism

Ipek Süntar, Esra Küpeli Akkol, Hikmet Keles, Erdem Yesilada,

Satyajit Sarker and Turhan Baykal

Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 06330, Ankara, Türkiye

Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200, Afyonkarahisar, Türkiye

Faculty of Pharmacy, Yeditepe University, Atasehir 34755, Istanbul, Türkiye

School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, Merseyside, United Kingdom

Abstract: Ethnobotanical surveys revealed that Daphne oleoidesSchreber has been used against rheumatic pain and for wound healing in Turkish folk medicine. The aim of the present study is to verify the folkloric assertion of D. oleoides ssp. oleoides (DOO) by bioassay-guided fractionation procedures leading to determination of the active component(s) and to elucidate the activity mechanisms of the isolated compounds. The wound healing activity of the methanol extract, its subextracts, fractions and isolates was evaluated by using two different in vivo wound healing experimental techniques . Anti-inflammatory and antioxidant activities of the test materials were also evaluated. For the determination of the activity mechanisms, the isolated compounds were screened for hyaluronidase, collagenase and elastase enzyme inhibitory activities. The methanolic extract of DOO was found to possess potent wound healing activity. This extract was then subjected to successive solvent extractions with n-hexane, dichloromethane, ethyl acetate (EtOAc) and n-butanol. EtOAc subextract yielded three compounds, quercetin 3-O-glucoside, triumbellin and rutarensin by using chromatographic separation techniques. The experimental study revealed that D. oleoides subsp. oleoides methanolic extract possesses significant wound healing effect and quercetin 3-O-glucoside was determined as the active component responsible from the activity.