12.

A New Acylated Flavonol Glycoside from Chenopodium foliosum

Zlatina Kokanova-Nedialkova, Magdalena Kondeva-Burdina, Dimitrina Zheleva-Dimitrova, Daniel Bücherl, Stefan Nikolov, Jörg Heilmann and Paraskev T. Nedialkov

Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, Dunav str. 2, 1000 Sofia, Bulgaria

Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, Dunav str. 2, 1000 Sofia, Bulgaria

Department of Pharmaceutical Biology, Institute of Pharmacy, University of Regensburg, 93053 Regensburg, Germany

Abstract: A new acylated flavonol glycoside, namely gomphrenol-3-O-( 5 '''-O-E-feruloyl)-β-D-apiofuranosyl-(1→2)[β-D-glucopyranosyl-(1→6)]-β-D-glucopyranoside (1) was isolated from the aerial parts of Chenopodium foliosum Asch. The structure of 1 was determined by means of spectroscopic methods (1D and 2D NMR, UV, IR, and HRESIMS). Radical scavenging and antioxidant activities of 1 were established using DPPH and ABTS radicals, FRAP assay and inhibition of lipid peroxidation (LP) in linoleic acid system by the ferric thiocyanate method. Compound 1 showed low activity (DPPH and ABTS) or lack of activity (FRAP and LP). In combination with CCl 4, 1 reduced the damage caused by the hepatotoxic agent and preserved cell viability and GSH level, decreased LDH leakage and reduced lipid damage. Effects were concentration dependent, most visible at the highest concentration (100 µg/m L ), and similar to those of silymarin .

Keywords: Flavonol triglycoside feruloyl ester; Chenopodium ; gomphrenol; Antioxidant and antihepatotoxic activity . © 2014 ACG Publications. All rights reserved.