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Chemical Constituents of Euphorbia Polyacantha Boiss. and their Immunomodulatory Properties
Gihan O. M. ELhassan, Achyut Adhikari, Omer M. Abdalla, Ahmed Shukrulla, Asaad Khalid, M. Iqbal Choudhary, Mohammed A. Mesaik, and Sakina Yagi
Department of Botany, Faculty of Science, University of Khartoum, Khartoum, Sudan
H. E. J. Research Institute of Chemistry, University of Karachi, Karachi-75270, Pakistan
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan
Institute of radiobiology, Sudan Atomic Energy Commission, Khartoum, Sudan
Medicinal and Aromatic Plants Research Institute, National Center for Research, Khartoum, Sudan
Medical Research Center, Jazan University, Jazan, Saudi Arabia
Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
Abstract: Phytochemical investigation on the stem of Euphorbia polyacantha Boiss. (Euphorbiaceae) afforded a new anthraquinone named as 1, 8-dihydroxy -3- (hydroxyl methyl) -5- methoxy anthracene- 9,10- dione (1) along with three known compounds, aloe emodin (2) , 3, 4, 3 ¢ –tri-O-methyl ellagic acid (3), and 3,3 ¢ ,4, 4 ¢ - tetra-O-methyl ellagic acid (4). Their structures were determined on the basis of IR, 1D- and 2D- NMR spectroscopy and mass spectrometry analysis. The effect of isolated compounds on oxidative burst of neutrophils and T-cell proliferation response was studied. Compounds 3 and 1 displayed strong oxidative burst inhibitory activity with IC 50 2 ± 0 and 3.5 ± 1.1 µg/mL respectively, whereas they moderately inhibited the T-cell proliferation with IC 50 28.5 ± 1.8 and 22.5 ± 2.0 µg/mL respectively. Compound 2 was relatively inactive with IC 50 43.2 μg/mL in oxidative burst and >50 μg/mL in case of T-cell proliferation. This is the first report on the phytochemical profile and biological activity of E. polyacantha.
Keywords:Euphorbia polyacantha; anthraquinone; immunomodulation; oxidative burst; T-cell proliferation. ©2015 ACG Publications. All rights reserved. |