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Composition, Antimicrobial and Antioxidant Properties of Endemic Species Ferulago macedonica Micevski & E. Mayer
Ksenija S. Mileski, Ana M. Džamić, Ana D. Ćirić, Mihailo S. Ristić, Slavica M. Grujić, Vlado S. Matevski and Petar D. Marin
University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 16, 1100 Belgrade, Serbia
University of Belgrade, Institute for Biological Research “Siniša Stanković“, Bul. despota Stefana 142,11000 Belgrade, Serbia
Institute for Medicinal Plant Research "Dr Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia
University “S. Kiril and Metodij” Faculty of Natural Sciences and Mathematics, Institute of Biology, “Gazi Baba”, 1000 Skopje, Macedonia
Macedonian Academy of Sciences and Arts, Bul. Krste Misirkov, 2, P.O. Box 428, 1000 Skopje, Macedonia
Abstract: Ferulago macedonica Micevski & E. Mayer (Apiaceae), an endemic species from Macedonia, was evaluated for its essential oil composition and biological activity for the first time. The essential oil composition from inflorescence and aerial parts, analyzed by gas chromatography and gas chromatography–mass spectrometry (GC and GC-MS), indicated α-pinene (43.10% and 22.78%) and sabinene (26.73% and 15.46%) as the main components. The antioxidant activities of extracts was determinated spectrophotometrically using 2,2-diphenyl-1-picrylhydrazyl ( DPPH). The strongest activity had methanol extracts of inflorescence and aerial parts (IC 50=490 mg/mL and IC 50=630 mg/mL of solution, respectively). According to 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) test, the most effective were ethanol and methanol extracts of F. macedonica inflorescence (1.69 and 1.63 mg Vit.C/g dw). Total phenolic and flavonoid contents evaluation indicated that their amounts were ranged between 34.12-59.68 mg gallic acid (GA)/g dw for phenols and 9.08 - 23.73 mg quercetin hydrate (QE)/g dw for flavonoids. Antimicrobial activity of extracts and essential oils was investigated by micro-well dilution assay. Minimum inhibitory (MIC) and minimum bactericidal/fungicidal concentrations (MBC/MFC) were defined. The methanol extract of inflorescence has shown to be the strongest in both, antibacterial ( MICs=4-8 mg/mL; MBCs=9-13 mg/mL ) and antifungal activity ( MICs=2-12 mg/mL; MFCs=6-18 mg/mL). Essential oil from inflorescence and aerial parts of F. macedonica manifested stronger inhibition on growth of pathogenic bacteria and fungy compared to tested extracts and positive standards.
Keywords: Ferulago macedonica; Apiaceae; essential oil; antioxidant activity; antimicrobial activity. © 2015 ACG Publications. All rights reserved. |