14.

A New Amide from the Stem Bark of Illiciumdifengpi and Its Anti-inflammatory Activity

Chuntong Li, Zhijun Wu and Wansheng Chen

Department of Pharmacy, Shanghai Changzheng Hospital, Second Military Medical University, Fengyang Road415, Shanghai 200003, China

Department of Pharmacy and Pharmacology, First Affiliated Hospital of PLA General Hospital, Beijing, 100048 China

Abstract:A new amide, named ( 2E, 4E)-5-phenyl-N-(2-phenylethyl)-2,4-pentadienamide (1) , together with one known amide, N -2-phenylet hylcinnamide (2) and two known ceramides, 2-​hydroxy-​N-​[(1S,​2R,​3E)​-​2-​hydroxy-​1-​(hydroxymethyl)​-​3-​heptadecenyl]​-pentadecanamide (3), 1-O-(β-D-glucopyranosyl)-(2S,3R,4E,8E)-2-[(2R)-2-hydroxypentadecanoylamino]-4,8-octadecadiene-1,3-diol (4) were isolated from the stem bark of Illicium difengpi. The structures of the isolated compounds were elucidated by analyses of their 1H and 13C NMR, COSY, H S QC, HMBC spectr a and HR-ESI/MS mass spectrometric data. Anti-inflammatory assays with compounds 1‒4 were carried out, compounds 1 and 2 showed significant inhibitory effect on TNF- α release in LPS stimulated RAW 264.7 macrophages .

Keywords: Amide; Illicium difengpi; anti-inflammatory . © 2015 ACG Publications. All rights reserved.