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A New Ceramide Along With Eight Known Compounds from the Roots of Artemisia incisa Pamp
Mamoon-Ur-Rashid, Muhammad Alamzeb, Saqib Ali, Ashfaq Ahmad Khan, John O Igoli, Valerie A Ferro, Alexander Irvine Gray and Mohammad Rafiullah Khan
Phytopharmaceutical and Nutraceutical Research Laboratories (PNRL), Institute of Chemical Sciences, University of Peshawar, Peshawar-25120, Pakistan;
Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, Scotland, UK; Department of Chemistry, University of Poonch Rawalakot, Jammu and Kashmir, Pakistan
Abstract: A new compound (1) (named as artemceramide-B) together with eight known compounds (taraxerol (2), taraxerol acetate (3), β-sitosterol (4), stigmasterol (5), trans-ethyl caffeate, dracunculin (7), scoparone (8) and isoscopoletin (9) were isolated from an ethanolic extract of the roots of Artemisia incisa Pamp (Asteracae). The structures of the compounds were determined through IR, 1D NMR ( 1H NMR, 13C NMR) and 2D NMR (COSY, NOESY, HSQC and HMBC) analyses. Accurate mass analyses were done with EI-MS, ESI-MS and acid methanolysis of compound 1 followed by GS-MS studies. The relative stereochemistry of artemceramide-B was determined by comparing its specific rotation and spectroscopic data with the literature. Compounds 1-9 were tested for their anti-bacterial potential against five bacteria strains; Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella pneumoniae, Bacillus subtilis and Escherichia coli. Compound 1 (new) (MIC: 0.0157, 0.0313 mg/mL ) and 7 (MIC: 0.0815 , 1.000 mg/mL ) showed excellent activities against S. epidermidis and S. aureus while compound 9 showed excellent activities (MIC: 0.0700 , 1.234, 1.890 and 2.286 mg/mL) against S. epidermidis,S. aureus, K. pneumoniae and E. coli, respectively. Compound 6 (MIC: 2.000 mg/mL) was found to be active against E. coli while neither of the compounds showed potential activity against B. subtilis.
Keywords: A. incisa; Asteraceae; artemceramide-B; methanolysis . © 2015 ACG Publications. All rights reserved. |