7.

Chemical Constituents, in vitro Antioxidant and Antiproliferative Activities of Perralderia coronopifolia Coss. subsp. eu-coronopifolia M. var. typica M. extract

Sara Boussaha, Khadidja Bekhouche, Azzedine Boudjerda, Francisco Leόn, Serkan Koldaş, Ayse Sahin Yaglioglu, İbrahim Demirtaş, Ignacio Brouard, Eric Marchioni, Djamila Zama, Samir Benayache and Fadila Benayache

Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Université Constantine 1, 25000 Constantine, Algérie

Instituto de Productos Naturales y Agrobiología, CSIC, Av. Astrofísico Fco. Sánchez, 3, 38206 La Laguna, Tenerife, Spain

Plant Research Laboratory, Chemistry Department, Çankırı Karatekin University, Ballica Campus, 18100 Çankırı, Türkiye

Equipe de Chimie Analytique des Molécules Bioactives (IPHC-LC4, UMR 7178), Universit é de Strasbourg, Facult é de Pharmacie, 74 Route du Rhin, 67400 Illkirch, France.

Abstract: Phytochemical investigations of extracts from the aerial parts (leaves and flowers) of Perralderia coronopifolia Cosson resulted in the isolation of nine secondary metabolites corresponding to three flavonoids: rhamnazin(1), chrysosplenol D (3), and (2R, 3R) taxifolin (4), two monoterpene glycosides : myrtenol- β-D-glucopyranoside- 6'-O-acetate (2) and myrtenol β-D-glucopyranoside (7), a disaccharide: sucrose (9) and three di-O-caffeoylquinic acid derivatives : methyl 3, 5-di-O-caffeoyl quinate (5 ) and methyl 3,4-di-O-caffeoyl quinate (6 ) as a mixture and 1,5-di-O-caffeoylquinic acid (8). The structures were identified by spectroscopic methods such as 1H and 13C NMR, COSY, HSQC and HMBC experiments, HRESI-MS and comparison with literature data. Myrtenol-β-D-glucopyranoside-6'-O-acetate (2) was isolated in pure and native state for the first time. The other compounds are new for the genus Perralderia Cosson. The ethyl acetate extract showed a high antioxidant effect, especially DPPH radical scavenging activity with IC 50=7.01±0.28µg/mL) compared to ascorbic acid ( IC 50= 5±0.1µg/mL . This extract also showed antiproliferative activity against HeLa (human cervix carcinoma) and C6 (rat brain tumor) cells.

Keywords: Asteraceae; Perralderia coronopifolia; flavonoids; caffeoylquinic acids; terpenoids; antioxidant activity; antiproliferative activity. © 2015 ACG Publications. All rights reserved.