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Neuroprotective and Antioxidant Constituents from Curcuma zedoaria Rhizomes

Omer Abdalla Ahmed Hamdi, Lo Jia Ye, Muhamad Noor Alfarizal Kamarudin, Hazrina Hazni, Mohammadjavad Paydar, Chung Yeng Looi, Jamil A. Shilpi, Habsah Abdul Kadir and Khalijah Awang

Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Biomolecular Research Group, Biochemistry Program, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Centre forNatural Products and Drug Discovery (CENAR), University of Malaya, 50603 Kuala Lumpur, Malaysia

Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia

Abstract: This study investigates the effect of phytochemical constituents from medicinally important plant Curcuma zedoaria (Christm.) Rosc., on hydrogen peroxide induced oxidative stress in mouse neuroblastoma-rat glioma hybridoma cells NG108-15. Phytochemical investigation of C. zedoaria rhizomes resulted in the isolation of nine sesquiterpenes (germacrone 1, dehydrocurdione 2, curcumenol 3, isoprocurcumenol 5, curcumenone 6, procurcumenol 7, zerumbone epoxide 8, zederone 9 and gweicurculactone 10) and one labdane diterpene (zerumin A 4). Curcumenol (3) and dehydrocurdione (2) showed 100% protection of the NG108-15 cells at the concentrations of 4 and 10 µM, respectively. Procurcumenol (7), isoprocurcumenol (5), zerumbone epoxide (8), zerumin A (4) and germacrone (1) showed moderate activity (80-90% protection). In the oxygen radical antioxidant capacity (ORAC) assay, all the test compounds showed strong antioxidant activity except curcumenol (3) which showed moderate antioxidant activity, as compared to the reference standard quercetin.

Keywords: Curcumenol; dehydrocurdion; zerumbone epoxide; ORAC assay; NG108-15 cells . © 2015 ACG Publications. All rights reserved.