Phytochemical Characterization of Filipendula ulmaria by UPLC/Q-TOF-MS and Evaluation of Antioxidant Activity
Milda Pukalskienė, Petras Rimantas Venskutonis and Audrius Pukalskas
Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų pl. 19, Kaunas LT-50254, Lithuania
Abstract: Antioxidant properties of Filipendula ulmaria (meadowsweet) extracts obtained with increasing polarity solvents, acetone, methanol and water were evaluated by the DPPH · and ABTS · + scavenging, oxygen radical absorbance capacity (ORAC) assays and by the content of total phenols. Methanol extract was screened by the on-line HPLC-UV-DPPH · scavenging assay, while phytochemical composition of extracts isolated at different plant vegetation phases was determined by HPLC-PDA-ESI-MS and UPLC-Q-TOF-MS 2. Eight compounds were identified and quantified, some other compounds were identified tentatively based on the obtained fragments and comparison with literature data . As a conclusion, the results demonstrate that F. ulmaria is a promising species for the development of novel high added value preparations .
Keywords: Filipendula ulmaria ; antioxidant activity; total phenolic compounds; flavonoids; plant vegetation stage. © 2015 ACG Publications. All rights reserved. |