11.

Characterization of one Novel Flavone and four New Source Compounds from the Bark of Millettia ovalifolia and In-Vitro Inhibition of Carbonic Anhydrase-II by the Novel Flavonoid

Taj Ur Rahman, Khanzadi Fatima Khattak, Wajiha Liaqat, Khair Zaman and Syed Ghulam Musharraf

Department of Chemistry, Abdul Wali Khan University, Mardan

Institute of Chemical Sciences, University of Peshwar-25120, Pakistan

International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi-75270, Pakistan

Abstract: The phytochemical examination of the extract of bark of Millettia ovalifolia yielded chemical constituents, which included one novel flavonoid 7-(4-methoxyphenyl)-9H-furo [2, 3-f] chromen-9-one and four new source compounds characterized as 3,7-Dihydroxy-2-phenyl-4H-chromen-4-one, (E)-Ethyl-13-(3,4-dimethoxyphenyl)acrylate, (E)-Methyl-3-(3,4-dimethoxyphenyl)acrylate and N-Ethylacetamide. These compounds were characterized by using advance modern spectroscopic analytical techniques such as UV, IR, 1D, 2D NMR and mass spectrometry. The novel flavonoid (1) displayed significant inhibition of cytosolic form of bovine carbonic anhydrase-II with IC 50 value of 17.86 ± 0.07 µM. This flavonoid may be used as a new pharmacophore to treat cystic fibrosis, glaucoma, epilepsy, leaukomia and other disorders such as neurology etc.

Keywords: Millettia ovalifolia bark; phytochemicals; spectral analysis; novel flavonoid; bovine carbonic anhydrase inhibition. © 2015 ACG Publications. All rights reserved.