Triterpenes from Meliosma oldhamii Miquel Branches and their Elastase Inhibitory Activities
Sang-Hee Byeon and Nam Ho Lee
Department of Chemistr y and Cosmetics , Jeju National University, Ara-1, Jeju 690-756, South Korea
Abstract: Phytochemical investigation o n the ethanol extracts of Meliosma oldhamii Miquel branches led to the isolation of seven triterpene constituents: betulin ( 1 ) , lupeol ( 2 ) , oleanolic acid ( 3 ) , 3 b -acetoxyolean-12-en-28-acid (4), 3 b -acetoxyolean-12-en-28-aldehyde (5), 3 b -acetoxy-28-hydroxyolean-12-ene (6) and maslinic acid ( 7 ) . Their chemical structures were determined based on the spectr oscopic studies, as well as by comparison with literature data. Elastase inhibition activities were examined for the isolates using ursolic acid as a positive control . In this test , the compounds 1 and 3 proved to inhibit porcine pancreatic elastase with an IC 50 values of 39.3 and 39.5 m g/mL, indicating comparable activities to ursolic acid (IC 50 = 28.5 m g/mL). This study demonstrated that the M. oldhamii extract including triterpenes has potentials applicable as anti-wrinkle ingredient in cosmetic formulations. All of the compounds 1 - 7 were isolated for the first time from M. oldhamii .
Keywords: Meliosma oldhamii ; isolation; triterpenes; elastase inhibition. © 2015 ACG Publications. All rights reserved. |