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Evaluation of Antioxidant, Cholinesterase Inhibitory and Antimicrobial Properties of Mentha longifolia subsp. noeana and Its Secondary Metabolites
Abdulselam Ertaş, Ahmet C. Gören, Nesrin Haşimi, Veysel Tolan and Ufuk Kolak
Department of Pharmacognosy, Faculty of Pharmacy, Dicle University, 21280 Diyarbakir, Turkey
TUBITAK UME, National Metrology Institute, Chemistry Group, Organic Chemistry Laboratory, 41470 Kocaeli, Turkey
Department of Nutrition and Dietetics, School of Health, Batman University, 72060 Batman, Turkey
Department of Biology, Faculty of Art and Science, Dicle University, 21280 Diyarbakır, Turkey
Department of General and Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34116 Istanbul, Turkey
Abstract: The aim of the present study was to determine the chemical structures of the isolated compounds, the essential oil and fatty acid compositions of Mentha longifolia subsp. noeana with their biological activities. Ursolic acid (1), u vaol (2), stigmast-5-ene-3 b -yl formate (3), stigmast-5-en-3-one (4), b -sitosterol (5), bis(2-ethylhexyl) benzene-1,2-dicarboxylate (6),hexacosyl (E)-ferulate (7) and 5-hydroxy-6,7,3',4'-tetramethoxy flavone (8) were obtained from the aerial parts. The compounds (2-4, 6, 7) were isolated for the first time from a Mentha species. Palmitic acid (40.8%) was the major component of the non-polar fraction obtained from the petroleum ether extract. Pulegone (32.3%) was the main constituent of the essential oil which exhibited strong butyrylcholinesterase inhibitory activity (77.36 ± 0.29%), moderate antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans. The methanol extract showed 80% inhibition of lipid peroxidation, and the acetone extract possessed moderate DPPH free radical scavenging activity (60% inhibition) at 100 m g/mL.
Keywords: Lamiaceae; M. longifolia subsp. noeana; essential oil; antioxidant; anticholinesterase. © 2015 ACG Publications. All rights reserved. |