12.

Essential Oil Contents and Micromorphological Traits of Stachys iva Griseb. and S. horvaticii Micevski (Lamiaceae)

Dario Kremer, Vlado Matevski, Valerija Dunkić, Nada Bezić and Edith Stabentheiner

Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, HR-10000 Zagreb, Croatia

Faculty of Natural Sciences and Mathematics, Ss Cyril and Methodius University, 1000 Skopje, Republic of Macedonia

Faculty of Science, University of Split, Teslina 12, HR-21000 Split, Croatia

Institute of Plant Sciences, Karl-Franzens University, Schubertstrasse 51, A-8010 Graz, Austria

Abstract: Chemical composition of the essential oil (EO) (analysed by GC and GC/MS), the types and distribution of trichomes, and pollen morphology were investigated in Stachys iva Griseb. and S. horvaticii Micevski (Lamiaceae) growing in Republic of Macedonia. The essential oil of Stachys iva was characterized by a high concentration of oxygenated sesquiterpene (43.8%) of which caryophyllene oxide (34.2%) and spathulenol (8.3%) being the principal compounds, while hexadecanoic acid being a major component in the both S. horvaticii oil with percentages of 25.7% in the oil from locality Babuna River Gorge and 28.4 % in the oil from locality Rajec River Gorge. Non-glandular trichomes, and two type of capitate trichomes (type 1 composed of one basal epidermal cell, one stem cell and a unicellular head cell with subcuticular space; type 2 composed of one elevated basal epidermal cell, one stem cells, and a head composed of four, sometimes two small cells) were observed on leaves, calyx and the stem.

Keywords: Caryophyllene oxide; hexadecanoic acid; pollen; Stachys iva; Stachys horvaticii; trichomes . © 2015 ACG Publications. All rights reserved.