Cytotoxic Activity of New Tropinene Glycoside Isolated from Solandra grandiflora Sw.

Magdy M. D. Mohammed, Michael Spiteller, Nabaweya A. Ibrahim and Khaled M. Mohamed

Institut für Umweltforschung (INFU), Technische Universität Dortmund, Otto-Hahn-Strasse 6, D-44221 Dortmund, Germany

Department of Pharmacognosy, National Research Centre, Dokki-12622, Cairo, Egypt

Abstract: The first phytochemical investigation of Solandra grandiflora Sw. leaves using LC/MS resulted in the identification without isolation of eight known and one unknown compounds from the neutral and the alkaloidal fractions. Further chromatographic fractionation and isolation of both fractions using RP-HPLC resulted in the isolation of chlorogenic acid, neochlorogenic acid, protocatechuic acid, scopoletin and isoscopoletin from the neutral fraction. Moreover, a new tropinene glycoside identified as 3-O-β-D- glucosyl 6-tropinene, together with atropine, scopolamine and hyoscyamine were isolated from the alkaloidal fraction. The structures of all isolated compounds were established and confirmed by 1H-, 13C-NMR, COSY, HSQC & HMBC spectroscopy, while the exact masses were confirmed by HRESIMS. The cytotoxic activity of all isolates was evaluated against HepG-2, HCT-116, A-549 and MCF-7, and the obtained results suggested selective antiproliferative and cytotoxic effect.

Keywords: Solandra grandiflora Sw.; Solanaceae; tropane alkaloids; cytotoxicity; SAR. © 2015 ACG Publications. All rights reserved.