3.

Variation in Scent Compounds of Oil-Bearing Rose (Rosa damascena Mill.) Produced by Headspace Solid Phase Microextraction, Hydrodistillation and Solvent Extraction

Sabri Erbaş and Hasan Baydar

Suleyman Demirel University, Faculty of Agriculture, Department of Field Crops, 32260, Isparta-Türkiye

Abstract: In this research, rose oil and rose water were hydro-distilled from the fresh oil-bearing rose flowers (Rosa damascena Mill.) using Clevenger-type apparatus. Rose concretes were extracted from the fresh rose flowers by using non-polar solvents, e.g. diethyl ether, petroleum ether, cyclo-hexane, chloroform and n-hexane, and subsequently by evaporation of the solvents under vacuum. Absolutes were produced from the concretes with ethyl alcohol extraction at -20°C, leaving behind the wax and other paraffinic substances. Scent compounds of all these products detected by gas chromatography (GC-FID/GC-MS) were compared with the natural scent compounds of fresh rose flower detected by using headspace solid phase microextraction (HS-SPME) with carboxen/polydimethylsiloxane (CAR/PDMS) fiber. A total of 46 compounds analysis were identified by HS-SPME-GC-MS in the fresh flower, and a total of 15 compounds were identified by GC-MS in the hydrodistilled rose oil. While main compounds in rose oil were geraniol (35.4%), citronellol (31.6%), and nerol (15.3%), major compound in fresh rose flower, rose water and residue water was phenylethyl alcohol (43.2, 35.6 and 98.2%, respectively). While the highest concrete yield (0.7%) was obtained from diethyl ether extraction, the highest absolute yield (70.9%) was obtained from the n-hexane concrete. The diethyl ether concrete gave the highest productivity of absolute, as 249.7 kg of fresh rose flowers was needed to produce 1 kg of absolute.

Keywords: Oil-bearing rose ; Rosa damascena; Distillation; Extraction; Volatile oil compounds; Headspace solid phase microextraction. © 2016 ACG Publications. All rights reserved.