6.

New Chemical Constituents from the Endophytic Fungus Xylariapapulis Cultivated on Taiwanese Lepidagathisstenophylla

Yi-Shuan Chen, Hsun-Shuo Chang, Ming-Jen Cheng, Hing -Yuen Chan, Ming -Der Wu, Sung-Yuan Hsieh, Hui-Chun Wang and Ih-Sheng Chen

Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan

School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan

Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan

Abstract: The endophytic strain Xylaria papulis was originated from the stem of Taiwanese Lepidagathis stenophylla C. B. Clarke ex Hayata (Acanthaceae) growing in the Hengchun Peninsula of southern Taiwan. Bioassay-guided fractionation of an EtOAc -soluble fraction of the liquid fermentate of this strain resulted in the isolation of two new isopimarane-type diterpene glycosides compounds, i.e., xylapapusides A & B (1 & 2), along with five previously identified compounds, 3–7. The structures of 1 and 2 were elucidated by detailed spectroscopic analysis including 1H- and 13C-NMR, COSY, HSQC, HMBC, and HR-ESI-MS, while the structures of the known compounds 3–7 were deduced from comparison of their spectral data with those in the literature. The effects of some isolates on the inhibition of NO production in lipopolysaccharide-activated RAW 264.7 murine macrophages were evaluated. Xylapapuside A (1) showed most potent of NO inhibition with E maxvalue of 34.3 μM.

Keywords:Xylaria papulis ; Lepidagathis stenophylla; endophytic fungus; isopimarane-type diterpene glycoside. © 2016 ACG Publications. All rights reserved.