Effects of Angelica Oil and the Isolated Butylphthalides on Glutamate-induced Neurotoxicity in PC12 Cells
Lu-Si Liu, Cheng Peng, Qin-Mei Zhou, Liang Xiong, Li Guo, Ya-Nan Wang and Ou Dai
State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Abstract: Angelica sinensis contains a large amount of essential oil (angelica oil), which is rich in phthalide derivatives with a lot of bioactivities. In vitro activity screening of angelica oil from the roots of A. sinensis found that it had concentration-dependent effect on glutamate-induced injury in PC12 cells. Further phytochemical investigation on this angelica oil led to the isolation of nine butylphthalides (1 –9) including two new compounds (1 and 2). Their structures were elucidated by extensive spectroscopic analyses. It is noteworthy that most of the isolated butylphthalides also displayed protective activity at low concentrations and cytotoxicity at high concentrations. These results imply that angelica oil and its main chemical components have protective effect for injured neurons only in appropriate concentration range.
Keywords: Angelica sinensis ; essential oil; butylphthalides; concentration-dependent effect; glutamate-induced injury; PC12 cells . © 2016 ACG Publications. All rights reserved.