Records of Natural Products

Year: 2017 Volume: 11   Issue: 3

 

  ORIGINAL ARTICLE

4.

Antioxidant, Antigenotoxic, Antimicrobial Activities and Phytochemical Analysis of Dianthus carmelitarum

Rezzan Aliyazicioglu, Selim Demir, Merve Badem, Sila Ozlem Sener, Nuriye Korkmaz, Elif Ayazoglu Demir, Ufuk Ozgen, Sengul Alpay Karaoglu and Yuksel Aliyazicioglu

Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Türkiye

Department of Nutrition and Dietetics, Faculty of Health Science, Karadeniz Technical University,

61080, Trabzon, Türkiye

Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University,

61080, Trabzon, Türkiye

Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Türkiye

Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdogan University, 53100, Rize, Türkiye

Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080,

Trabzon, Türkiye

Abstract: In this study, we investigated the phytochemical composition, antioxidant, antimicrobial, and antigenotoxic properties of the aqueous extract of Dianthus carmelitarum for the first time. The phenolic and volatile compounds, antioxidant, antimicrobial and antigenotoxic activities of the extract were determined by HPLC and SPME-GC-FID/MS, spectrophotometric, agar well diffusion methods and comet assay, respectively. The polyphenolic content and ferric reducing power values of the extract were found 12.6 ± 0.27 mg gallic acid and 238 ± 2.89 μM trolox equivalents per g sample, respectively. Syringaldehyde and chlorogenic acid were detected as major phenolic compounds, while terpenes were determined as major volatile compound. Dianthus carmelitarum extract especially exhibited moderate antimicrobial activity against Mycobacterium smegmatis. Extract reduced H 2O 2-induced DNA damage in a concentration dependent manner in fibroblast cells compared to positive control (only 20 μM H 2O 2 treatment). Dianthus carmelitarum can be considered in the food, cosmetic, and drug industries due to its antioxidant, antimicrobial, and antigenotoxic activities.

 Key words: Antimicrobial activity; antioxidant effect; chromatography; Dianthus carmelitarum; genotoxic stress.

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