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New 5-oxomilbemycins from a Genetically Engineered Strain Streptomyces bingchenggensis BCJ60

Jiansong Li, Hui Zhang, Shaoyong Zhang, Haiyan wang, Ji Zhang, Anliang Chen, Jidong Wang and Wensheng Xiang

Life Science and Biotechnology Research Center, School of Life Science, Northeast Agricultural University, Harbin 150030, China

Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin’An 311300, China

Department of New Drug Screening, Zhejiang Hisun Pharmaceutical Co., Ltd, Taizhou 318000, China

Abstract: Two new 5-oxomilbemycins, 27-aldehyde -5-oxo milbemycin β 12 (1) and 2-hydroxymilbemycin K (2) , were isolated from a genetically engineered strain Streptomyces bingchenggensis BCJ60. Their structures were determined by comprehensive analyses of its 1H and 13C NMR, COSY, HMQC, and HMBC spectroscopic, HR - E S I - MS mass spectrometric and comparison with data from the literature . Preliminary studies showed that 27-aldehyde -5-oxo milbemycin β 12 (1) and 2-hydroxymilbemycin K (2) possessed strong acaricidal and nematocidal activities.

Keywords: New 5-oxomilbemycins ; genetically engineered strain ; Streptomyces bingchenggensis BCJ60; acaricidal and nematocidal activities. © 2017 ACG Publications. All rights reserved.