6.

Melilotoside Derivatives from Artemisia splendens (Asteraceae)

Fariba H. Afshar, Abbas Delazar, Hossein Nazemiyeh, Lutfun Nahar, Sedigheh B. Moghaddam, Blessing O. Mbaebie, Simon Gibbons and Satyajit D. Sarker

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

Medicinal Chemistry and Natural Products Research Group, School of Pharmacy and Biomolecular Sciences, Liverpool Jhon Moores University, James Parsons Building, Byron Street, Liverpool L3 3AF, UK

UCL School of Pharmacy, 29-39 Brunswick Square WC1N 1 AX, London, UK

Abstract: A combination of solid-phase-extraction (SPE) and reversed-phase preparative high-performance liquid chromatography (prep-HPLC) of the methanolic extract of the aerial parts of Artemisia splendens (common name: “Asia Minor Wormwood”), an endemic Iranian species, afforded Z- and E-melilotosides (1 and 2), Z- and E-4-methoxy-melilotosides (3 and 4), and a new dimer, bis-ortho-Z-melilotoside (5, named: splendenoside). Whilst the structures of these compounds (1-5) were elucidated unequivocally by spectroscopic means, the in vitro free-radical-scavenging property of 1-5 was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. This is the first report on the occurrence of any melilotoside derivatives in the genus Artemisia.

Keywords: Artemisia splendens; Asteraceae; 2,2-diphenyl-1-picrylhydrazyl (DPPH); free-radical scavenger; melilotoside; splendenoside. © 2016 ACG Publications. All rights reserved.