Records of Natural Products

Year: 2017 Volume: 11   Issue: 6




The Flavonoid Fraction from Rhoeo discolor Leaves Acts Antiviral Against Influenza A Virus

Yazmin Sánchez-Roque, Guadalupe Ayora-Talavera, Reiner Rincón-Rosales, Federico Antonio Gutiérrez-Miceli, Rocío Meza-Gordillo, Robert Winkler, Roberto Gamboa-Becerra, Teresa del Rosario Ayora-Talavera and Víctor Manuel Ruiz-Valdiviezo

Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana km 1080, C.P. 29050, Tuxtla Gutiérrez, Chiapas, México

Laboratory of Virology, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán. Av. Itzaes 490, C.P. 97000, Mérida, Yucatán, México

CINVESTAV, Unidad Irapuato, Dept Biochem & Biotechnol, Km 9-6 Libramiento Norte Carretera Irapuato-León 36821 Km 9.6, C.P.36821, Irapuato, Guanajuato, México

Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Unidad Sureste. Km 5.5 Sierra Papacal-Chuburná Puerto, 97302, Mérida, Yucatán, México

Abstract:I n vitro antiviral effect of a crude methanol extract and six fractions isolated from Rhoeo discolor against pandemic influenza A (H1N1) evaluated herein. The phytochemical analysis to identify the main classes of secondary metabolites was performed by thin layer chromatography (TLC). The cytotoxic effect and cell viability was determined in MDCK cells, and the antiviral activity was evaluated by qRT-PCR to detect influenza virus nucleoprotein (NP) gene. Finally a metabolomic analysis was performed using UPLC-MS. The phytochemical analysis revealed the presence of coumarins, tannins, saponins and flavonoids in the methanol extracts. Cytotoxicity related to 50% cell viability indicated concentrations of <1μg/mL for the six fractions tested and >1μg/mL for the crude extracts. Fraction MF1 inhibited synthesis of viral NP at co-treatment level at a concentration of 0.02 + 0.07 g/mL with a selectivity index (SI) of 32. The metabolomic analysis identified the presence of five flavonoids kaempferol, quercetin, isoquercetin luteolin-5-glucoside and rutin. Antiviral activity of flavonoids from R. discolor against the influenza AH1N1 virus are reported for the first time.

Keywords: Antiviral agent; flavonoids; in vitro; hemagglutinin; real-time PCR; metabolite . © 2017 ACG Publications. All rights reserved.