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Chemical and Biological Investigation of Ochrosia elliptica Labill. Cultivated ‎in Egypt

Riham A. El-shiekh, Dalia A. Al-Mahdy, Mohamed S. Hifnawy, Tzvetomira Tzanova, ‎Emilie Evain-‎Bana, Stéphanie Philippot, Denyse Bagrel and Essam A. Abdelsattar

Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr El Aini st., P.B. 11562, Egypt

Molecular Engineering Laboratory and Pharmacological Biochemistry (LIMBP), Jean Barriol Institute, University of Lorraine, Metz, France

Abstract: The phytochemical investigation of Ochrosia eliptica leaves resulted in isolation and identification of eight compounds; lupeol, lupeol acetate, uvaol, ursolic acid, β-sitosterol glucoside, rutin, 8-methoxy and 9-methoxy ellipticine . The ethanolic extract and fractions were studied for their cytotoxic, antioxidant and anti-inflammatory activities. The cytotoxic activity was performed on human mammary adenocarcinoma (MCF7), its multidrug-resistant ‎ counterpart (VCREMS), estrogen receptor negative human metastatic breast ‎ adenocarcinoma cells (MDA-MB-231) and the non-cancerous, immortalized by ‎ telomerase, human breast epithelial cell line (hTERT-HME1). Additionally, the inhibitory potential on Cdc25s proteins was determined. The results showed that the dichloromethane (DCM) fraction and the major alkaloid; 9-methoxyellipticine exhibited high inhibitory activity against all tested cell lines particularly MCF7 and VCREMS cell lines, whereas t he DCM fraction showed a significant inhibitory action on Cdc25 A isoform. In contrast, the n-butanol fraction and 9-methoxyellipticine displayed the highest antioxidant potential. The DCM fraction showed significant anti-inflammatory activity compared to indomethacin. This work comprises the first comprehensive work to be conducted on O. elliptica leaves showing its potential in multiple biological activities .

Keywords: Ochrosia elliptica ; methoxy ellipticine; cytotoxicity; Cdc25s; anti-inflammatory. ‎ © 2017 ACG Publications. All rights reserved.