Chemical Composition, C ytotoxic , Antimicrobial and Antioxidant Activities of Essential oil from Anthriscus caucalis M. Bieb Grown in China Pengxiang Lai , Huijuanzi Rao and Yang Gao Marine College, Shandong University, Weihai 264209, China Abstract: The essential oil of the aerial parts of Anthriscus caucalis M.Bieb was obtained by hydrodistillation and its components were analy z ed by using GC and GC-MS. Forty-six compounds representing 97.2% of the total oil were identified. The main constituents in the oil were identified as β-Bisabolene (28.4%), Germacrene D (18.9%), (Z, E)-α-Farnesene (16.8%) and γ-Muurolene (7.3%). I n vitro cytotoxicity evaluation against two cell lines of HepG2 (liver hepatocellular cells) and MCF-7 (human breast adenocarcinoma) cells showed a potent cytotoxic activity with the IC 50 values of 67.50 μg/mL and 55.83 μg/mL according to the MTT assay. Furthermore, t he essential oil exhibited a considerable activity against Bacillus subtilis and Escherichia coli with the MIC values of 0.095 mg/mL and 0.105 mg/mL tested by micro-dilution method . The antioxidant activity was evaluated by DPPH and FRAP methods, and the essential oil gave an IC 50 value of 0.451 mg/mL and a Trolox equivalent antioxidant concentration of 191.7 ± 11.3 μmol Trolox × g -1 in DPPH and FRAP, respectively. The results indicated that the essential oil was relatively active and may be useful in food and pharmaceuticals after more detailed study. Keywords: Anthriscus caucalis M. Bieb; essential oil; antioxidant activity; antibacterial activity; cytotoxic activity. © 2018 ACG Publications. All rights reserved |