Antioxidant and Neuroprotective Activity of the Aerial Parts of Seven Eragrostis Species and Bioactive Compounds from E. japonica
Chae Sun Na, Min Ju Lee , Seong Su Hong, Yun-Hyeok Choi, Jae-Eun Lee, So-Young Park, Yong Ho Lee and Sun Hee Hong
Institute of Ecological Phytochemistry, Department of Plant Life and Environmental Science, Hankyong National University, Anseong 456-749, Gyeonggi-do, Republic of Korea
Institut für Botanik, Leopold-Franzens-Universität-Innsbruck, Sternwartestraße 15, 6020 Innsbruck, Austria
Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Suwon 16229, Republic of Korea
Laboratory of Pharmacognosy, College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea
Seed bank of Wild Resource Plants, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea
Abstract: The main purpose of this study was to evaluate the antioxidant and neuroprotective activities in the aerial parts of seven Eragrostis species (Poaceae) and to find antioxidant or neuroprotective compounds from the most active species. The total phenolic content (TPC), total flavonoid content (TFC), 1,1-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH RSA), total antioxidant capacity (TAC), and neuroprotective activity against amyloid beta peptide induced toxicity in PC12 cells were measured in the methanol extracts of the aerial parts of E. ferruginea, E. cilianensis, E. minor, E. multicaulis, E. pilosa, E. japonica, and E. curvula collected from June to August 2013. All species showed antioxidant or neuroprotective activities and, among them, E. japonica was the most active species to isolate antioxidant or neuroprotective compounds, because it was found to show both the highest TPC (10.63 ± 0.31 mg/g) and TFC (2.83 ± 0.06 mg/g) values, as well as TAC (11.34 ± 0.80 mg/g) and DPPH RSA (47.07 ± 2.81 µg/mL), with the second-highest neuroprotective value (23.0 µg/mL). Three known compounds were isolated from E. japonica by the bioassay guided approach and these were identified as isoorientin, isovitexin, and caffeic acid that have antioxidant and neuroprotective activities.
Keywords: Eragrostis species; Eragrostis japonica; antioxidant activity; neuroprotective activity. © 2017 ACG Publications. All rights reserved.