Isolation and Antimicrobial Activity of Rutin and Its Derivatives from Ruta chalepensis (Rutaceae) Growing in Iraq Shaymaa Al-Majmaie, Lutfun Nahar, George P. Sharples, Khazal Wadi and Satyajit D. Sarker Medicinal Chemistry and Natural Products Research Group, School of Pharmacy and Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool L3 3AF, United Kingdom Department of Biology, College of Science, University of Diyala, Baquba, Iraq Abstract: Rutin (1), rutin 3'-methyl ether (2), and a new flavonol glycoside, 6-hydroxy-rutin 3',7-dimethyl ether (3), were isolated from the methanol extract of the fruits of Ruta chalepensis , collected from Diyala, Iraq. Their structures were elucidated by spectroscopic analyses, including 1D-, 2D-NMR and HR ESI MS. Antimicrobial activity of compounds 1-3 was tested against four Gram +ve and Gram –ve bacterial strains, and the only fungal strain, Candida albicans, using the 96-well based resazurin microtitre assay. Keywords: Ruta chalepensis ; Rutaceae; resazurin assay; antimicrobial activity; rutin ; 6-hydroxy-rutin 3‵,7-dimethyl ether . © 2018 ACG Publications. All rights reserved. |