JOURNAL 1298


Records of Natural Products
VOLUME & ISSUE
Available Online: August 23,2019
PAGES
p.1 - 9
DOI ADDRESS
http://doi.org/10.25135/rnp.144.19.06.1298
(DOI number will be activated after the manuscript has been available in an issue.)
STATISTICS
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AUTHORS
  • San Yoon Nwe
  • Chayapol Tungphatthong
  • Areerat Laorpaksa
  • Boonchoo Sritularak
  • Witchuda Thanakijcharoenpath
  • Somboon Tanasupawat
  • Suchada Sukrong
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


Paphiopedilum callosum (Rchb.f.) Stein belongs to the Orchidaceae family. The phytochemistry and bioactivities of this plant have not been reported. In this study, a bioassay-guided isolation with a yeast cell-based assay was performed to isolate topoisomerase I poison compounds from the roots of P. callosum. One new and five known compounds were isolated. According to their spectroscopic data (ESI-MS, NMR, IR and UV), the new compound (1) is 3′-hydroxy-2,6,5′-trimethoxystilbene. The known compounds (2-6) were identified by comparing their spectroscopic data with those in the literature. According to the yeast cell-based assay, all the compounds were topoisomerase Ι poisons based on their ability to inhibit the growth of yeast cells. As further confirmation, the cytotoxic activities of the isolated compounds were evaluated using human cancer cell lines (MCF-7 and NCI-H187). The compounds exhibited varying degrees of cytotoxicity on the representative cell lines. These isolated compounds could serve as new lead compounds in the development of cancer chemotherapeutics.

KEYWORDS
  • Paphiopedilum callosum
  • Orchidaceae
  • yeast cell-based assay
  • topoisomerase Ι poison
  • stilbene

SUPPORTING INFORMATION


Supporting Information
Download File 144-RNP-1906-1298-SI.pdf (959.29 KB)