JOURNAL


Records of Natural Products
VOLUME & ISSUE
Available Online: April 30,2019
PAGES
p.1 - 8
DOI ADDRESS
http://doi.org/10.25135/rnp.125.18.12.1132
(DOI number will be activated after the manuscript has been available in an issue.)
STATISTICS
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AUTHORS
  • Zhenggang Yue
  • Rui Zhou
  • Yihan He
  • Hongbo Xu
  • Yalei Pan
  • Liyan Lei
  • Pei Xie
  • Zhishu Tang
  • Jinao Duan
PDF OF ARTICLE

ABSTRACT


Pseudolarix amabilis (Nelson) Rehd, is a monotypic genus plant belonging to the family Pinaceae. The root bark , known as “Tu-Jin-Pi” has been used for the treatment of skin diseases. During our activity screening, the water-soluble part of the 70% EtOH extract of P. amabilis bark showed excellent inhibitory bioactivities on PTP1B enzyme, leading to the phytochemical isolation of the root bark of P. amabilis. Three oleanane-type compounds (1-3) and seven phenolic compounds (4-10) were isolated, in which oleanolic acid 3-O-β-D-glucuronyl-6′-ethyl ester (1) was identified as a new saponin. The chemical structures of these compounds were elucidated by 1D/2D nuclear magnetic resonance and high resolution mass spectra. In addition, their pharmacological inhibitory bioactivities on PTP1B enzyme were evaluated, and the three oleanane-type compounds 1-3 exhibited inhibitory bioactivities with IC50 values of 1.90 ± 0.37, 19.15 ± 0.10 and 10.44 ± 0.59 μM, respectively.

KEYWORDS
  • Pseudolarix amabilis
  • PTP1B inhibitors
  • Isolation and identification
  • Oleanane-type compounds
  • Phenolic compounds
  • Oleanolic acid 3-O-β-D-glucuronyl-6′-ethyl ester

SUPPORTING INFORMATION


Supporting Information
Download File 125-RNP-1812-1132-SI.pdf (7.13 MB)