JOURNAL 2025


Records of Natural Products
VOLUME & ISSUE
Available Online: June 07,2021
PAGES
p.1 - 10
DOI ADDRESS
http://doi.org/10.25135/rnp.259.21.03.2025
(DOI number will be activated after the manuscript has been available in an issue.)
STATISTICS
Viewed 28 times.
AUTHORS
  • Nichakan Peerakam
  • Prapart Phoowiang
  • Sunee Chansakaow
  • Chalermporn Thongpoon
  • Sudarat Hadpech
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


This study we aimed to characterize the chemical and biological properties of the edible plant, Clausena harmandiana (Pierre) Guillaumin. Fresh leaves of this plant were hydrodistilled to obtain the essential oil, which was characterized by GC-MS. Total phenolic content was evaluated by Folin-Ciocalteau assay. Biological activity was assessed by measuring antioxidants, antimicrobial activities, and the inhibitory effects on tyrosinase, collagenase, and a-glucosidase. The primary components were trans-anethole (91.44%), followed by estragole (2.98%) and cis-anethole (2.55%). The essential oil contained the amount of phenolic 20.63 mg GAE/g extract and showed the activity to scavenge DPPH (IC50 18.48 mg/mL), ABTS (IC50 3.97 mg/mL) radicals and also presented the ability to reduce Fe3+-TPTZ complex on FRAP assay (3.74 mg TEAC/g extract). Additionally, the C. harmandiana essential oil exhibited antimicrobial activity against Candida albicans (MIC 6.63 mg/ml, MFC 13.25 mg/mL) and interestingly presented enzymatic inhibitory effects on collagenase (IC50 0.059 mg/mL) and a-glucosidase (IC50 0.677 mg/mL). The remarkable activity of inhibition on a-glucosidase was two times more to acarbose (IC50 1.26 mg/mL).

KEYWORDS
  • Clausena harmandiana
  • Rutaceae
  • chemical profile
  • biological activity

SUPPORTING INFORMATION


Supporting Information
Download File 259-RNP-2103-2025-SI.pdf (1.23 MB)