JOURNAL 3165


Records of Natural Products
VOLUME & ISSUE
Available Online: April 30,2024
PAGES
p.1 - 8
DOI ADDRESS
http://doi.org/10.25135/rnp.454.2403.3165
(DOI number will be activated after the manuscript has been available in an issue.)
STATISTICS
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AUTHORS
  • Shihao Zhang
  • Minghua Ma
  • Sicheng Liu
  • Guoce Jin
  • Wencong Li
  • Yu Zhao
  • Yanyu Li
  • Xueyan Li
  • Zixuan Zhu
  • Chengxiong Liu
  • Zhaoxia Liu
  • Xiaocong Li
  • Kun Zou
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


Three crude polysaccharides of RCP-A, B, and C were derived from the rhizome of Rohdea chinensis by means of hot water extraction, gradient ethanol precipitation and dialysis. Three different polysaccharides of RCP-C1-1, RCP-C1-2, and RCP-C1-3 were isolated using cellulose DEAE-52 and Sephadex G-200 chromatography from RCP-C. The average molecular weights of RCP-C1-1, RCP-C1-2, and RCP-C1-3 were measured as 1.51×104, 1.06×104, and 4.86×103 by means of MALDI-TOF MS and UHPGC, respectively. All three polysaccharides were found to consist of D-fructose and D-glucose following hydrolysis and comparison with literature data. Based on FT-IR and NMR analysis, the polysaccharides were identified as inulin-type fructans, with their backbone composed of α-D-glucopyranosyl-(1→2)-(β-D-fructofuranosyl)n-(1→2)-β-D-fructofuranoside (nRCP-C1-1=79, nRCP-C1-2=58, nRCP-C1-3=27). The anti-tumor activity of the polysaccharides (RCP-A, B, and C) was evaluated in H22 tumor-bearing mice. The results suggested that the polysaccharides (RCP-A, RCP-B, and RCP-C) inhibited the growth of H22 hepatocellular. Further, the treated groups of RCP-A, RCP-B, and RCP-C exhibited improvements in body weight as well as spleen/thymus indexes in H22 tumor-bearing mice.

KEYWORDS
  • Rohdea chinensis
  • polysaccharides
  • anti-tumor activity
  • inulin-type fructans

SUPPORTING INFORMATION


Supporting Information
Download File 454-RNP-2403-3165-SI.pdf (1.21 MB)