Validated spectrophotometric methods for simultaneous estimation of Paracetamol , Domperidone and Tramadol HCl in pure and tablet dosage form

Two methods are developed for simultaneous estimation of paracetamol, domperidone and tramadolHCl in pure and tablet dosage form by using 0.1N NaOH as a solvent. Paracetamol, domperidone and tramadol HCl show absorbance maximums at 256 nm, 289.6 nm and 218.4 nm respectively. Shimadzu UV 1700, capable of multicomponent analysis, was used for quantitation. In method I, absorbance of the sample solution was measured at 256 nm and 289.6 nm for the estimation of paracetamol, domperidone respectively as tramadol HCl does not absorb at these wavelengths. Estimation of tramadol HCl was carried out at 218.4 nm. Method II is based on a multiwavelength spectroscopic method. Validation study reveals that the methods are specific, accurate, precise, and reproducible. All three drugs obey Beer's law in the concentration ranges used for the methods. Validation studies are statistically significant as all the statistical parameters are within the acceptance range (% COV< 2.0 and S.D. < 2.0) for both accuracy and precision study. High recovery and low % COV reveals the reliability of the method for quantitative study of three drugs in tablet formulation. The methods are simple, rapid accurate, precise, reproducible, and economic and can be used for routine quantitative analysis of paracetamol, domperidone and tramadol HCl in pure and tablet dosage form.