Journal of Chemical Metrology

A scientific open access journal in the field of analytical chemistry and accreditation
Editor-in-Chief: Mustafa Özyürek
Book Review and Review Article Editor: John Warren

LATEST ARTICLES

Original Article

Primary level electrolytic conductivity measurements at National Metrology Institute of Turkey (TUBITAK UME)

J. Chem. Metrol. (2020) in press ; 1 - 10
by Emrah Uysal , Lokman Liv , Fehmi Fıçıcıoğlu and Mehedin Arifoviç

TUBITAK UME (National Metrology Institute of Turkey) Electrochemistry Laboratory established the primary level electrolytic conductivity measurement system in 2018. The objective of this work is to prove the accuracy of the established system with an acceptable uncertainty and to disseminate the traceability for electrolytic conductivity measurements. While the accuracy of the system was tested with the certified conductivity solutions, the accuracy of the software developed at TUBITAK UME was also tested. The cell constant was determined as 0.57986 cm-1 at 25 °C by using certified conductivity solutions. The accuracy of the system was evaluated using the measurement of certified conductivity solutions and it was found that there was no significant difference between the results at 95% confidence level. Finally, “Evaluation of measurement data - Guide to the expression of uncertainty in measurement” and “EA-4/02 M: 2013 Evaluation of the Uncertainty of Measurement in Calibration” guides were used to form the uncertainty budget for primary level electrolytic conductivity measurements.

DOI
http://doi.org/10.25135/jcm.34.20.01.1524
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Electrolytic conductivity jones cell CRM traceability
Available online: March 24, 2020
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© ACG Publications. All rights reserved.
Original Article

Liquid chromatographic analysis for the determination of deferasirox in pharmaceutical formulations and spiked plasma samples using dansyl chloride reagent

J. Chem. Metrol. (2020) in press ; 1 - 7
by Ali Asram Sagiroglu , Cem Onal and S. Evrim Kepekçi Tekkeli

In the current study, a new sensitive and precise method based on HPLC and derivatization with dansyl chloride was developed for quantification of deferasirox (DEF) in pharmaceutical dosage forms and spiked plasma samples. Separation procedure in the chromatographic system was performed using a mobile phase consisting of the mixture of methanol and acetic acid solution (0.5 M, pH adjusted to 7.0 with NaOH) with a ratio of 70:30 v/v at flow rate of 1.0 mL per minute under isocratic elution on a C18 column (150 mm × 4.6 mm, 5 μm I.D.). The excitation wavelength was selected as 340 nm and emission wavelength was selected as 480 nm for the measurement of the analyte signal. The retention time for DEF is approximately 3.5 min. ICH Guidelines were taken into account for method validation. For the deferasirox concentration range of 20–2000 ng/mL, the proposed analytical method exhibited a linear relationship between the drug concentration and measured fluorescence with a correlation coefficient of 0.9994.The currently developed method can be implemented efficiently for the quantification of DEF in pharmaceutical dosage forms and spiked plasma samples.

DOI
http://doi.org/10.25135/jcm.35.20.01.1518
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Deferasirox HPLC method validation pharmaceutical formulation spiked plasma
Available online: March 24, 2020
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© ACG Publications. All rights reserved.