Journal of Chemical Metrology

A scientific open access journal in the field of analytical chemistry and accreditation
Editor-in-Chief: Mustafa Özyürek
Book Review and Review Article Editor: John Warren

LATEST ARTICLES

Original Article

Simultaneous quantification of teneligliptin hydrobromide and metformin hydrochloride: An improved HPTLC method with implementation of Plackett-Burman design

J. Chem. Metrol. (2021) in press ; 1 - 11
by Mehul Patel , Divya Patel , Umang Shah and Hetaben M. Kachhiya

Simple, rapid, sensitive, robust, and validated high-performance thin-layer chromatography was developed for the simultaneous determination of anti-diabetic drugs Teneligliptin hydrobromide (TH) and Metformin hydrochloride (MH) in bulk and pharmaceutical formulation. HPTLC system equipped Camag Linomat V, TLC Scanner IV, Twin Trough chamber, Dual-wavelength UV Cabinet, and win CATS software V1.4.7 were used during the study. Separation of analytes were achieved using a pre-coated silica gel G60 F254 aluminum Sheets 10×10 cm2, with the thickness of 0.2 mm as stationary phase and a mobile phase comprising of Methanol: Ammonium sulfate (0.5 %w/v): Triethylamine (9: 2.7: 0.5 v/v/v). The detection wavelength was set at 237 nm. The Rf value of the TH and MH was 0.63 and 0.19, respectively. The method was validated using a dynamic linear range of  4-28 ng/band and 100-700 ng/band for TH and MH, respectively. The developed method was validated as per the ICHQ2 (R1) guideline. The statistical analysis proved that the method is accurate with 98.80–99.38 % w/v and 99.66–99.96 % w/v for TH and MH, respectively, and precise with less than 2 % RSD. The Plackett-Burman design was applied to check the robustness of the developed method. Finally, the method is termed as linear, precise, accurate, and robust. It can be implemented successfully for the routine analysis of pharmaceutical dosage forms comprising TH and MH and is appropriate for regulatory submission under regulatory flexibility.

DOI
http://doi.org/10.25135/jcm.55.20.11.1893
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
: Teneligliptin hydrobromide metformin hydrochloride high-performance thin-layer chromatography ICH guidelines simultaneous estimation quality by design
Available online: January 22, 2021
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Original Article

Measurement uncertainty evaluation for titrimetric determination of benzoic acid purity

J. Chem. Metrol. (2021) in press ; 1 - 14
by Lokman Liv

The uncertainty budget for the determination of benzoic acid purity by potentiometric titrimetry was proposed. For this purpose alcoholic potassium hydroxide solution was standardized with benzoic acid supplied from National Institute of Standards and Technology (NIST) (SRM 39j), then the purity of the benzoic acid sample was determined by the same way. Measurement uncertainty evaluation of the method was exhaustively evaluated according to the EURACHEM/CITAC Guide CG 4, JCGM 100: 2008 and EUROLAB Technical Report No.1/2007, based on the bottom-up approach. Moreover, these results were compared with the results based on the top-down approach according to the EUROLAB Technical Report No.1/2007 and NORDTEST: NT TR 537. The measurement uncertainty was separately evaluated for the factor determination and the purity determination of one and six samples of benzoic acid for the bottom-up approach. The uncertainty sources for the purity determination of benzoic acid were carbon dioxide mole fraction, nominal concentration of benzoic acid, factor of alcoholic potassium hydroxide, amount of titrant consumption, the molar mass of benzoic acid and sample amount, respectively. Among these, the highest contributions to the uncertainty budget were found to be the factor of alcoholic KOH solution and the amount of titrant consumption for one and six samples, respectively. The expanded uncertainties for one and six measurements based on the bottom-up approach were found as 0.508% and 0.509%, and the expanded uncertainty for six measurements based on the top-down approach was found as 0.114%.

DOI
http://doi.org/10.25135/jcm.54.20.11.1881
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Benzoic acid traceability electrochemistry measurement uncertainty bottom-up approach top-down approach
Available online: January 11, 2021
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Original Article

A comparative view to heavy metal pollution in soil and rainwater in Çanakkale, Turkey

J. Chem. Metrol. (2021) in press ; 1 - 14
by Sibel Mentese , Ozlem Tonguc Yayintas , Batuhan Bas , Latife Ceyda İrkin and Selehattin Yilmaz

On Earth, atmosphere interact with crust and thus simultaneously monitoring of environmental pollution in both parts of the environment is important. Soil and rainwater samples were taken in different parts of Çanakkale, Turkey, in two seasons. Study sites laid along with the prevailing wind direction (from NE to SW) as wind has the potential to distribute pollutants emitted into the air throughout its path.  The concentrations of selected elements were determined by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Also, physical parameters such as pH, temperature, electrical conductivity of the rainwater and temperature and pH value of the soil samples were measured together with the meteorological parameters. Seasonal differences for the selected elements were insignificant in the soil samples (p>0.05), while some elements showed seasonal variations in the rainwater samples (p<0.05). The highest average heavy metal levels were found for Zn > Pb > Mn> Cu> Cd > Ni> Cr in the rainwater samples and Mn > Zn > Cu > Pb > Cr > Ni > Co in the soil samples, respectively. The highest enrichments were found for Pb in the rainwater and As in the soil samples. Elevated As levels occurred in the samples can pose a great risk for public health and agriculture.

DOI
http://doi.org/10.25135/jcm.52.20.09.1817
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Atmospheric pollution Çanakkale ICP-MS meteorological parameters rainwater soil pollution
Available online: December 21, 2020
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Original Article

Increasing the production of some specific cytotoxic triterpenoids and evaluation of the morpho-physiological response associated with in vitro salt stress in Pistacia khinjuk Stocks.

J. Chem. Metrol. (2021) in press ; 1 - 12
by Emine Ayaz Tilkat

Triterpenoids are a class of secondary metabolites that are known to exhibit and possess various biological activities. Using plant biotechnological tools, a novel approach for producing these compounds offers opportunities not only to produce such bioactive secondary metabolites but also to increase the quantities of these essential phytochemicals. Therefore, this study was aimed to elucidate the biosynthesis of some cytotoxic triterpenoids through in vitro micropropagation of Pistacia khinjuk Stocks with the application of different NaCl concentrations (0 to 250 mM) and to evaluate the triterpenoids content as well as synchronize the morpho-physiological properties of plant materials during the salt application. The effects of these salt concentrations were investigated on P. khinjuk seedlings grown for four weeks by using the MS medium supplemented with 100 mg/L L-ascorbic acid and NaCl of given concentrations. LC-MS/MS results showed that the amount of all triterpenoid types from different plant parts was increased while root-shoot lengths, RWC, total Chl, Chl-a/b, and carotenoid values were decreased significantly at high salinity concentrations (150 mM). The reduction of the quantitative and qualitative morpho-physiological parameters was due to the production of an increased amount of secondary compounds, i.e., triterpenoids rather than primary compounds in response to the increased salt concentrations.

DOI
http://doi.org/10.25135/jcm.53.20.10.1883
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Pistacia khinjuk stocks LC-MS/MS alt stress triterpenoid in vitro plant physiology
Available online: December 20, 2020
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