Records of Natural Products

A scientific open acces journal in the field of natural products.
Editor-in-Chief: Gülaçtı Topçu
Honorary Editor: Ayhan Ulubelen
Co-Editor-in-Chief: Ahmet C Gören

LATEST ARTICLES

Original Article

The Efficacy of Gynostemma pentaphyllum Extract in Anti-obesity Therapy

Rec. Nat. Prod. (2019) in press ; 1 - 13
by Yoon Hee Kim , So Mi Kim , Jae Kyoung Lee , Sung Kwan Jo , Hyung Joong Kim , Kyu Min Cha , Cho Young Lim , Joo Myung Moon , Tae Young Kim and Eun Ji Kim

In the present study, we investigated a saponin-enriched fraction from Gynostemma pentaphyllum extract (GPE) and isolated gypenoside L (GL), gypenoside LI (GLI), and ginsenoside Rg3 (Rg3). To explore the anti-obesity activities of these compounds, we investigated the effects of GL, GLI, and Rg3 on glucose uptake and activation of AMPK in L6 skeletal muscle cells. GPE, GL, GLI, and Rg3 markedly increased glucose uptake activity and GLUT4 gene expression in L6 myotubes. The enhanced glucose uptake was mediated by the activation of the AMPK-ACC signaling pathway. In addition, we observed that GPE, GL, GLI, and Rg3 increased the expression of SIRT1 and PPARγ coacvtivator-1α (PGC-1α). Our results suggest the potential use of Gynostemma pentaphyllum in developing a therapeutic agent to reduce body weight and prevent diet-related diseases via glucose uptake and AMPK activation.

DOI
http://doi.org/10.25135/rnp.146.19.05.1270
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Gynostemma pentaphyllum gypenosides L6 skeletal muscle cells glucose uptake AMPK SIRT1
Available online: August 23, 2019
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Original Article

Bioassay Guided Isolation of Topoisomerase Ι Poison from Paphiopedilum callosum (Rchb.f.) Stein

Rec. Nat. Prod. (2019) in press ; 1 - 9
by San Yoon Nwe , Chayapol Tungphatthong , Areerat Laorpaksa , Boonchoo Sritularak , Witchuda Thanakijcharoenpath , Somboon Tanasupawat and Suchada Sukrong

Paphiopedilum callosum (Rchb.f.) Stein belongs to the Orchidaceae family. The phytochemistry and bioactivities of this plant have not been reported. In this study, a bioassay-guided isolation with a yeast cell-based assay was performed to isolate topoisomerase I poison compounds from the roots of P. callosum. One new and five known compounds were isolated. According to their spectroscopic data (ESI-MS, NMR, IR and UV), the new compound (1) is 3′-hydroxy-2,6,5′-trimethoxystilbene. The known compounds (2-6) were identified by comparing their spectroscopic data with those in the literature. According to the yeast cell-based assay, all the compounds were topoisomerase Ι poisons based on their ability to inhibit the growth of yeast cells. As further confirmation, the cytotoxic activities of the isolated compounds were evaluated using human cancer cell lines (MCF-7 and NCI-H187). The compounds exhibited varying degrees of cytotoxicity on the representative cell lines. These isolated compounds could serve as new lead compounds in the development of cancer chemotherapeutics.

DOI
http://doi.org/10.25135/rnp.144.19.06.1298
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Paphiopedilum callosum Orchidaceae yeast cell-based assay topoisomerase Ι poison stilbene
Available online: August 23, 2019
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Short Report

A New Record of the Marine Red Alga Laurencia snackeyi from Japan and its Chemotaxonomic Significance

Rec. Nat. Prod. (2019) in press ; 1 - 4
by Takahiro Ishii , Wakana Hisada , Tsuyoshi Abe , Norio Kikuchi and Minoru Suzuki

The constitution of Laurencia snackeyi (Weber-van Bosse) Masuda (Ryukyu-sozo in Japanese) from three locations, Minna Island, Sesoko Island and Kayo Coast in Okinawa Prefecture, Japan, is reported. This species produced snyderane-type sesquiterpenes, palisadin A (1), palisadin B (2) and aplysistatin (3) as characteristic metabolites. The species name of "Laurencia luzonensis Masuda", which has previously been reported in several papers, should be revised to L. snackeyi.

DOI
http://doi.org/10.25135/rnp.145.19.06.1310
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Laurencia snackeyi Laurencia luzonensis Rhodomelaceae chemotaxonomy snyderane-type sesquiterpene
Available online: August 23, 2019
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Original Article

Phytochemical Investigation of Endemic Sideritis cypria Post

Rec. Nat. Prod. (2019) in press ; 1 - 11
by Duygu Yiğit Hanoğlu , Azmi Hanoğlu , Hasan Yusufoğlu , Betül Demirci , Kemal Hüsnü Can Başer , İhsan Çalış and Dudu Özkum Yavuz

Non-polar and polar compounds were isolated from the leaves and chemical composition of the essential oil from aerial parts of Sideritis cypria endemic in Northern Cyprus was also determined. The structures of all the isolated compounds were elucidated by means of spectroscopic (UV, NMR: 1D and 2D-NMR: 1H, 13C, COSY, HSQC, HMBC) methods and comparison with literature data. The essential oil composition of the plant material was analysed by GC-FID and GC-MS, simultaneously. To the best of our knowledge, secondary metabolites of this endemic species were determined for the first time. As a result, two mixtures of four ent-kaurane diterpenes; sidol (1) and isosidol (2), linearol (3) and isolinearol (4); four phenylethanoid glycosides; verbascoside (5), lavandulifolioside (6), leonoside A (7), leucoseptoside A (8); four flavone glycosides, apigenin-7-O-glucopyranoside (9), isoscutellarein-7-O-[6'''-O-acetyl-allopyranosyl-(1->2)-glucopyranoside] (10) and a mixture of apigenin-7-O-(4''-O-p-coumaroyl)-glucopyranoside (11) and apigenin-7-O-(3''-O-p-coumaroyl)-glucopyranoside (12) were elucidated. 7-O-acetyl-8-epi-loganic acid (13), an iridoid glycoside, was reported herein for the first time for genus Sideritis. In addition, major compounds of the essential oil were determined as a-pinene (14.73±0.15%), b-pinene (16.60±0.20%), b-phellandrene (17.83±0.23%) and epi-cubebol (7.70±0.20%), respectively.

DOI
http://doi.org/10.25135/rnp.140.18.11.1079
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Sideritis Phytochemistry ent-kaurane diterpenes iridoids flavonoids essential oil
Available online: August 01, 2019
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