Records of Natural Products
A scientific open acces journal in the field of natural products.
Records of Natural ProductsA scientific open acces journal in the field of natural products.
Chemical Composition, Enantiomeric Distribution and AChE-BChE Activities of the Essential Oil of Myrteola phylicoides (Benth) Landrum, from Ecuador
The volatile constituents of the essential oil (EO) of Myrteola phylicoides (Benth) Landrum, from Ecuador, extracted by steam distillation have been analyzed. A total of 37 compounds, representing 90.30% the total essential oil sample were identified. Monoterpenes hydrocarbons (53.06%) and sesquiterpene hydrocarbons (35.24%) were the principal groups of compounds. The major components were identiﬁed as α-pinene (30.94%), (E)-caryophyllene (21.93%), β-pinene (14.45%) and α-humulene (9.56%). The essential oil of M. phylicoides showed weak in vitro activity against AChE inhibition with IC50 value 60.8 μg/mL and a low BChE activity with IC50 value >250 μg/mL. This is the first report on the chemical composition of the essential oil of this specie.DOI http://doi.org/10.25135/rnp.112.18.09.893 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Myrteola phylicoides essential oil AChE BChE Available online: December 15, 2018 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.
Cytotoxicity of Some Plants of the Asteraceae Family: Antiproliferative Activity of Psiadia punctulata Root Sesquiterpenes
According to the World Health Organization Cancer is the second leading cause of death globally. The methanol extracts of fourteen Middle-Eastern plants of the family Asteraceae were screened for antiproliferative activity on five cancer cell lines (A2780, MCF7, HeLa, RKO and Jurkat) by using MTT assay. Psiadia punctulata (DC.) Vatke was selected for isolation and elucidation of the bioactive constituents by 1D- and 2D-NMR, and MS analyses. Flow cytometry was used to evaluate cell cycle analysis, apoptotic hallmarks and reactive oxygen species. P. punctulata yielded a new sesquiterpene characterized as 7-hydroxy eudesman-3,5-dien-2-one (punctulin) (3) and three known sesquiterpenes: 1, 2 and 4. The antiproliferative activity of all sesquiterpenes was evaluated in Jurkat (T-cell leukemia) and HeLa cancer cell lines. Compound 1 (1β-hydroxy-8-oxo-cyperone) has induced a significant growth inhibition in Jurkat and HeLa cells (IC50 =12 and 18µM respectively). Flow cytometry of compound 1 has elucidated the mechanism of action by showing its ability to induce cell cycle arrest in Jurkat cells mainly in G0/G1 and, less markedly, in G2/M. Compound 1 also expressed strong antioxidant activity by reducing the basal level of peroxides DHFDA-load in Jurkat cells. Compound 1 antioxidant activity may have contributed to the observed cell cycle arrest.DOI http://doi.org/10.25135/rnp.184.108.40.2069 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Cytotoxic Activity Asteraceae Leukemia Cell cycle Sesquiterpenes Available online: December 15, 2018 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.
Chemical Composition and Biological Activities of the Essential Oil from Aristolochia fordiana Hemsl
Abstract: The present study investigated the chemical composition of the essential oil obtained from the aerial parts of Aristolochia fordiana Hemsl (AF-EO) using GC-FID and GC-MS, and evaluated the in vitro biological activities of the essential oil. Forty-nine compounds representing 99.6% of the total oil were characterized. The main constituents were identified as β-chamigrene (17.0%), β-caryophyllene (11.1%), α-bulnesene (11.0%) and β-pinene (10.2%). Furthermore, the antibacterial activity of the essential oil of A. fordiana was studied using disc diffusion and micro-broth dilution assays. AF-EO exhibited a significant antibacterial activity against Staphylococcus aureus and Bacillus subtilis with MIC values below 100 μg/mL. Besides, the results of MTT assays indicated that the essential oil exhibited a moderate cytotoxic activity on HepG2 (liver hepatocellular cells) and MCF-7 (human breast adenocarcinoma cells) cell lines. However, the AF-EO showed a weak antioxidant activity in DPPH•, ABTS•+ and FRAP assays.DOI http://doi.org/10.25135/rnp.111.18.09.897 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Aristolochia fordiana Hemsl essential oil antibacterial activity cytotoxic activity antioxidant activity Available online: November 28, 2018 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.
Assessment of Endemic Cota fulvida (Asteraceae) for Phytochemical Composition and Inhibitory Activities against Oxidation, -Amylase, Lipoxygenase, Xanthine Oxidase and Tyrosinase Enzymes
In the present work, chemical compositions of essential oil and methanol extract of endemic Cota fulvida (Grierson) Holub were investigated as well as their antioxidant, antidiabetic, antiinflammatory and antimelanogenesis potent. The phytochemical analyses have been performed with GC-MS/FID and LC-MS/MS techniques. The essential oil was characterized with hexadecanoic acid (25.6 %), camphor (6.1 %), caryophyllene oxide (5.3 %), 1,8-cineole (4.9 %) and humulene epoxide (3.9 %). In the extract, phenolic acids, phenylpropanoid dimer and flavonoids were detected. The major constituents of the extracts were found to be 5-feruloylquinic acid, caftaric acid, 3,5-O-dicafeoylquinic acid and quercetin rutinoside. The antioxidant activities of the oil and extract were evaluated through scavenging of free radicals, inhibition of linoleic acid peroxidation and superoxide anion radical (O2-) generated by xanthine - xanthine oxidase (XO) system. The extract showed free radical scavenging activity (IC50 0.131 mg/mL), Trolox equivalent antioxidant capacity (1.33 mM) and inhibited (Inh. 36.3 %) peroxidation of lipids. The oil and extract demonstrated significant hypoglycemic activity via inhibition of porcine pancreatic a-amylase. The antiinflammatory effects of the oil and extract via inhibition of 5-LOX enzyme were found as 53.7 % and 23.9 %, respectively. The extract demonstrated moderate inhibitory effect (23.3 %) on oxidation of L-DOPA via inhibition of tyrosinase enzyme.DOI http://doi.org/10.25135/rnp.109.18.09.875 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Cota fulvida Essential Oil Extract GC-MS/FID LC-MS/MS Antioxidant Available online: November 15, 2018 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.