Records of Natural Products
A scientific open acces journal in the field of natural products.
Records of Natural ProductsA scientific open acces journal in the field of natural products.
Inhibition of iNOS Induction and NF-κΒ Activation by Taste Compounds from the Edible Mushroom Tricholoma caligatum (Viv.) Ricken
Tricholoma caligatum (Viv.) Ricken is an edible mushroom that belongs to matsutake group. The first chemical investigation of the three different extracts of Tricholoma caligatum resulted in two new compounds, 8-demethoxylascivol (1) and 8-epi-lascivol (2) and six known compounds, lascivol (3), trametenolic acid (4), ergosterol (5), ergosterol peroxide (6), 5a, 6a-epoxyergosterol (7), and cerebroside B (8). Their structures were elucidated by spectroscopic analyses including 1D and 2D NMR data. The biological activities of all the compounds were evaluated toward multiple targets related to inflammation and metabolic disorder such as NF-κΒ, iNOS and ROS. The findings of this study reveal that the edible mushroom Tricholoma caligatum could be a potential source for anti-inflammatory bioactive metabolites.DOI http://doi.org/10.25135/rnp.139.19.04.1263 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Tricholoma caligatum iNOS NF-κΒ anti-inflammatory lascivol Available online: June 22, 2019 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.
Comparative Effects of Flavonoids from Fructus Sophorae on Rat Osteoblasts in vitro
Fructus Sophorae (FS), the dry mature fruit of Sophora japonica Leguminosae, is a valuable traditional Chinese medicine resource with flavonoids as the major active ingredients. To identify the plant-derived estrogen-like flavonoids serving as potential osteoporosis chemopreventive agents, we isolated and identified 27 flavonoids compounds, including 17 compounds obtained for the first time from FS. To screen out the flavonoids with estrogen-like biological behavior from the 11 high yield compounds, we set up an in vitro screening system in rat osteoblast MC3T3-E1 cells including MTT assay, alkaline phosphatase staining and Alizarin red S staining assay to examine the effects on cell proliferation, differentiation and mineralization of osteoblast cells respectively. Six flavonoids, including genistein, sophoricoside, sophorabioside, sophoraflavonoloside, nicotiflorin and rutin, significantly increased the cellular activity of MC3T3-E1 cells. Furthermore, blocking the estrogen receptor signaling by tamoxifen compromised the effects above significantly, suggesting the 6 compounds behave as estrogen-like reagents. Moreover, the interaction between the six flavonoids from FS and estrogen receptor was clarified by molecular docking method from Glide XP. Collectively, being used as food and medicine in China, FS is rich in flavonoids with estrogen-like effects, may be used as healthy supplementary in treating postmenopausal women osteoporosis.DOI http://doi.org/10.25135/rnp.138.19.04.1262 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Fructus Sophorae Flavonoid Phytoestrogens Estrogen Receptor Osteoporosis Available online: June 22, 2019 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.
Two Novel Sesquiterpenes and A New Pregnane Derivative from the South China Sea Gorgonian Subergorgia suberosa
Two novel sequiterpenes isosuberosenol A (1), suberosain A (2), and a new pregnane derivative 4-hydroxymethyl-5β-pregnan-3, 20-dione (3) have been isolated from the South China Sea gorgonian Subergorgia suberosa. Their structure and relative stereochemistry were established based on the detailed spectroscopic analysis, including HR-ESI-MS, 1D- and 2D NMR spectra. The skeleton of isosuberosenol A (1) have been previously undescribed. The discovery of isosuberosenol A (1) provides some strong support for the plausible biogenetic pathway of quadrone type sesquiterpenes.DOI http://doi.org/10.25135/rnp.137.19.04.1257 (DOI number will be activated after the manuscript has been available in an issue.) Keywords sesquiterpene pregnane derivative Subergorgia suberosa Available online: June 22, 2019 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.
Bioassay-guided Isolation and Identification of Anti-inflammatory Sesquiterpene Lactones from Chrysophthalmum montanum (DC.) Boiss.
The aerial parts of Chrysophthalmum montanum (DC.) Boiss. (Asteraceae) is traditionally used for wound healing, as well as for the treatment of common cold, sinusitis, and other inflammatory diseases. The objectives of this study were to identify potential anti-inflammatory effects of the methanol extract, and its different polarity subextracts (n-hexane, chloroform, n-butanol, and remaining aqueous), and guaianolide-type sesquiterpene lactones [6α-acetoxy-4α-hydroxy-1βH-guaia-9.11(13)-dien-12.8α-olide (1), 6α-acetoxy-4α-hydroxy-9β.10β-epoxy-1βH-guaia-11(13)-en-12.8α-olide (2), 4α,6α-dihydroxy-1β,5α,7αH-guaia-9(10),11(13)-dien-12,8α-olide (3), and (4α,5α,8β,10β)-4,10-dihydroxy-1,11(13)-guaidien-12,8-olide (4)] from the aerial parts of C. montanum. In order to evaluate the anti-inflammatory activity of C. montanum, carrageenan- and PGE2-induced hind paw edema, and acetic acid-induced increase in capillary permeability mice models were used. The methanol extract, the chloroform subextract, and compounds 3, and 4 were shown to possess anti-inflammatory activity in in vivo models at 100 mg/kg dose. The results provide a biological, and phytochemical basis for the traditional use of C. montanum aerial parts for inflammatory conditions in Turkish folk medicine.DOI http://doi.org/10.25135/rnp.220.127.116.114 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Asteraceae Chrysophthalmum montanum sesquiterpene lactones anti-inflammatory carrageenan prostaglandins Available online: June 16, 2019 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.