Records of Natural Products
A scientific open acces journal in the field of natural products.
Records of Natural ProductsA scientific open acces journal in the field of natural products.
Alkaloid Profiling of Hippeastrum cultivars by GC-MS, Isolation of Amaryllidaceae Alkaloids, and Evaluation of Their Cytotoxicity
Different species, hybrids and varieties of the genus Hippeastrum (Amaryllidaceae) are planted for ornamental purposes and at the same time they represent a rich source of interesting secondary metabolites called Amaryllidaceae alkaloids. These compounds display a wide spectrum of biological activities. In the current study, six Hippeastrum taxa were evaluated for their alkaloid profile by GC-MS. Using preparative TLC, five alkaloids were isolated in pure form, and three of them evaluated for cytotoxic and antiproliferative potencies on a panel of human cancer cells of different histotype (Jurkat, MOLT-4, A549, HT-29, PANC-1, A2780, HeLa, MCF-7 and SAOS-2). In parallel, normal MRC-5 human fibroblasts were employed to determine the compounds´ overall toxicity against non-cancer cells. The most intriguing cytotoxic profile was demonstrated by montanine (1) with IC50 values between 1.04 – 1.99 µM.DOI http://doi.org/10.25135/rnp.147.19.06.1302 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Amaryllidaceae Hippeastrum alkaloids montanine cytotoxicity Available online: September 11, 2019 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.
A new 2,2′-dipyridine and Its Analogues from Endophytic Streptomyces sp. KIB H017c with Potent Cytotoxicity
A new 2,2′-dipyridyl compound 1, along with three known analogues (2–4), was isolated from the culture broth of endophytic Streptomyces sp. KIB H017c derived from Fragaria ananassa. Their structures were elucidated by analysis of 1D and 2D NMR as well as HRESIMS data, and comparison with literature data. The biological properties of all isolates were explored for antibacterial, antifungal and cytotoxic activity. All four compounds exhibited weak antifungal activity against Saccharomyces cerevisiae, and compound 4 exhibited weak antifungal activity against Penicillium decumbens ATCC 10436. Compound 3 displayed moderate cytotoxic activity against HL-60 (human myeloid leukemia), SMMC-7721 (hepatocellular carcinoma), A-549 (lung carcinoma), MCF-7 (breast adenocarcinoma), and SW480 (colon carcinoma) with IC50 values of 12.10, 11.15, 23.64, 31.13 and 14.62 μM, respectively. Compound 4 exhibited potent activity against A-549 (lung carcinoma), MCF-7 (breast adenocarcinoma), and SW480 (colon carcinoma) with IC50 values 0.88, 0.34 and 0.55 μM, respectively.DOI http://doi.org/10.25135/rnp.147.19.05.1268 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Endophytic Streptomyces 2,2′-dipyridine antimicrobial activity cytotoxicity Available online: September 11, 2019 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.
The Efficacy of Gynostemma pentaphyllum Extract in Anti-obesity Therapy
In the present study, we investigated a saponin-enriched fraction from Gynostemma pentaphyllum extract (GPE) and isolated gypenoside L (GL), gypenoside LI (GLI), and ginsenoside Rg3 (Rg3). To explore the anti-obesity activities of these compounds, we investigated the effects of GL, GLI, and Rg3 on glucose uptake and activation of AMPK in L6 skeletal muscle cells. GPE, GL, GLI, and Rg3 markedly increased glucose uptake activity and GLUT4 gene expression in L6 myotubes. The enhanced glucose uptake was mediated by the activation of the AMPK-ACC signaling pathway. In addition, we observed that GPE, GL, GLI, and Rg3 increased the expression of SIRT1 and PPARγ coacvtivator-1α (PGC-1α). Our results suggest the potential use of Gynostemma pentaphyllum in developing a therapeutic agent to reduce body weight and prevent diet-related diseases via glucose uptake and AMPK activation.DOI http://doi.org/10.25135/rnp.146.19.05.1270 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Gynostemma pentaphyllum gypenosides L6 skeletal muscle cells glucose uptake AMPK SIRT1 Available online: August 23, 2019 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.
Bioassay Guided Isolation of Topoisomerase Ι Poison from Paphiopedilum callosum (Rchb.f.) Stein
Paphiopedilum callosum (Rchb.f.) Stein belongs to the Orchidaceae family. The phytochemistry and bioactivities of this plant have not been reported. In this study, a bioassay-guided isolation with a yeast cell-based assay was performed to isolate topoisomerase I poison compounds from the roots of P. callosum. One new and five known compounds were isolated. According to their spectroscopic data (ESI-MS, NMR, IR and UV), the new compound (1) is 3′-hydroxy-2,6,5′-trimethoxystilbene. The known compounds (2-6) were identified by comparing their spectroscopic data with those in the literature. According to the yeast cell-based assay, all the compounds were topoisomerase Ι poisons based on their ability to inhibit the growth of yeast cells. As further confirmation, the cytotoxic activities of the isolated compounds were evaluated using human cancer cell lines (MCF-7 and NCI-H187). The compounds exhibited varying degrees of cytotoxicity on the representative cell lines. These isolated compounds could serve as new lead compounds in the development of cancer chemotherapeutics.DOI http://doi.org/10.25135/rnp.144.19.06.1298 (DOI number will be activated after the manuscript has been available in an issue.) Keywords Paphiopedilum callosum Orchidaceae yeast cell-based assay topoisomerase Ι poison stilbene Available online: August 23, 2019 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.