Records of Natural Products

Articles In Press

Original Article

1) A New Acylated Benzyl Alcohol Glucoside from Syzygium austroyunnanense

Rec. Nat. Prod. (2018) in press ; 1 - 5
by Feng Li, Shaohua Xu, Yan Zhao, Mengjia Li, Wen Xu, Yikao Hu, Dingli Zhang and Yong Zhao
Abstract

A new acylated benzyl alcohol glucoside, benzyl alcohol 3',6'-di-O-galloyl-b-glucopyranoside (1), together with a known analogue, 6'-O-galloyl-b-glucopyranoside (2)  and a cyanogenic glucoside, 6‵-O-galloylprunasin (3) were isolated from the leaves of Syzygium austroyunnanense. Their structures were characterized based on the spectroscopic methods and comparison with literature. This is the first phytochemical study on Syzygium austroyunnanense.

DOI
http://doi.org/10.25135/rnp.85.18.04.277
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Syzygium austroyunnanense Myrtaceae acylated glycoside.
Available online: September 25, 2018
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Original Article

2) Composition of the Essential Oils of Two Endemic Helichrysum Species in Turkey

Rec. Nat. Prod. (2018) in press ; 1 - 7
by Mine Kürkçüoğlu, Hale Gamze Ağalar, Ahmet Aksoy, and Kemal Hüsnü Can Başer
Abstract

The aerial parts of Helichrysum noeanum Boiss. and H. chionophilum Boiss. et Balansa were hydrodistilled for 3 h using a Clevenger-type apparatus. The essential oils were analyzed by GC-FID and GC-MS, simultaneously. The main constituents were identified as hexadecanoic acid (25.3%), dodecanoic acid (17.8%), tetradecanoic acid (13.7%), and decanoic acid (4.2%) for H. noeanum. Decanoic acid (18.7%), tetradecanoic acid (16.9%), dodecanoic acid (13.8%), hexadecanoic acid (12.2%) and hexahydrofarnesyl acetone (3.2%) were found to be the major compounds for H. chionophilum. Several reports have been encountered in the literature dealing with the oil composition of several Helichrysum species. To the best of our knowledge, the essential oil of H. chionophilum has not previously been investigated.

DOI
http://doi.org/10.25135/rnp.93.18.06.113
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Helichrysum noeanum H. chionophilum essential oil GC GC-MS.
Available online: September 20, 2018
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Original Article

3) New Cytotoxic Metabolites from Pathogenic Fungus Cylindrocarpon destructans Associated with Meconopsis grandis

Rec. Nat. Prod. (2018) in press ; 1 - 7
by Dilfaraz Khan, Shah Iram Niaz, Kamran Tahir, Hidayat Ullah Khan, Muhammad Haroon, Shahid Ullah Khan, Syed Badshah, Shafiullah Khan, Muafia Jabeen and Liu Lan
Abstract

Phytochemical investigation on pathogenic fungus Cylindrocarpon destructans isolated form Meconopsisgrandis plant led to the isolation of two new isochromene derivatives namely, 6,8-dimethoxy-3-methyl-3,4-dihydrobenzoisochromene-4,9,10-triol (1) and 3,5,6-trihydroxy-4-methylbenzoisochromene-9,11-dione (2) along with four known compounds (3-6). The structures of these compounds were elucidated by 1D and 2D NMR and mass spectroscopic data analysis. The isolated compounds were evaluated for cytotoxic activity. The compounds 1-4 showed good inhibition against the growth of cell lines MCF-7 and PC-3. Compounds 5-6 showed minimum inhibitory effect of cancerous cell lines growth.

DOI
http://doi.org/10.25135/rnp.67.18.01.207
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Pathogenic fungus Cylindrocarpon destructans Meconopsis grandis cytotoxic activity
Available online: August 27, 2018
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Original Article

4) In vitro Propagation and Volatile Compound Characterization of Lavandula stoechas L. subsp. stoechas- An Economically Important Source of Essential Oil

Rec. Nat. Prod. (2018) in press ; 1 - 8
by Sam Mokhtarzadeh, Betül Demirci, Hale Gamze Ağalar,Khalid Mahmood Khawar and Nese Kırımer
Abstract

The objective in this study is to isolate and characterize volatile compounds of Lavandula stoechas L. subsp. stoechas rooted with micropropagation techniques and grown under suitable in vitro conditions. Microdistillation procedure was applied on aerial parts of this plant. The volatile compounds were characterized by GC-FID and GC-MS systems, simultaneously. Based on results, twenty-two compounds were identified. Major volatile compounds included camphor (38.5%), bornyl acetate (10.6%), α-fenchone (8.9%), 1,8-cineole (4.3%), α-pinene (4.0%), linalool (3.5%), viridiflorol (3.8%) myrtenal (2.7%), geranyl acetate (2.1%). These results were positively related with data published regarding the plant growing wild.

DOI
http://doi.org/10.25135/rnp.86.18.04.105
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Lavandula stoechas subsp. stoechas tissue culture microdistillation volatile compounds.
Available online: August 04, 2018
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Original Article

5) Diterpenoid Alkaloids from the Roots of Aconitum sinomontanum and Their Evaluation of Immunotoxicity

Rec. Nat. Prod. (2018) in press ; 1 - 9
by Jiao Zhang, Yuze Li, Yuwen Cui, Pu Jia, Zhenggang Yue, Bei Song and Xiaomei Song
Abstract

A new C 18-diterpenoid alkaloid, along with four known diterpenoid alkaloids have been isolated from the roots of Aconitum sinomontanum. Their structures were established as sinomontanine I (1), delcosine (2), lepenine (3), napelline (4), and kirinine B (5) by extensive spectroscopic techniques and chemical methods. The immunosuppressive effects of compounds 1 –4 were evaluated in vitro through ConA-induced or LPS-induced splenocyte proliferation, with IC 50 values of 8.909 μM, 1.515 μM , 5.078 μM, and 1.167 μM ( ConA-induced ) , or 3.661 μM, 4.417 μM, 5.129 μM, and 1.830 μM (LPS-induced), and compounds 1 –4 showed a significant cytotoxic effect with CC 50 values of 447.5 μM, 702.2 μM, 310.6 μM and 794.1 μM, respectively. The CC 50/IC 50 value of 2 and 3 suggested that these compounds were potential immunosuppressive agents for the treatment of autoimmune diseases characterized by arthritis, such as rheumatoid arthritis.

DOI
http://doi.org/10.25135/rnp.89.18.05.296
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Aconitum sinomontanum Nakai diterpenoid alkaloids immunotoxicity
Available online: August 04, 2018
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Original Article

6) Wound-Healing Activity of Some Species of Euphorbia L.

Rec. Nat. Prod. (2018) in press ; 1 - 10
by Wound-Healing Activity of Some Species of Euphorbia L.
Abstract

 Some species of Euphorbia have been used as medicinal plants to treat wounds, and skin diseases, around the world. The solvents n-hexane, ethyl acetate, and methanol were used successively to prepare extracts of the aerial parts of E. characias subsp. wulfenii, E. helioscopia,E. macrocladaE. seguieriana subsp. seguieriana, and E. virgata. Linear incision, circular excision wound models and the hydroxyproline assay method were used to assess the wound-healing activity. The inhibition of the increase in capillary permeability induced by acetic acid was used to assay the anti-inflammatory activity. The methanol extract of the aerial parts of E. characias subsp. wulfenii showed statistically significant wound-healing activity with 43.03% tensile strength for the linear incision wound model and a 65.24% reduction in the area of the wound by day 10 for the circular excision model. The tissue treated with this extract was found to contain 35.47 µg/mg of hydroxyproline. The methanol extract of E. characias subsp. wulfeniiinhibited inflammation induced by acetic acid with a value of 34.74%. The results showed that the aerial parts of E. characias subsp. wulfenii possess wound-healing and anti-inflammatory activities on different models.

DOI
http://doi.org/10.25135/rnp.81.18.03.255
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Euphorbia anti-inflammatory excision incision wound-healing .
Available online: July 18, 2018
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Erratum

7) Erratum to “ Headspace Analysis of Volatile Compounds Coupled to Chemometrics in Leaves from the Magnoliaceae Family

Rec. Nat. Prod. (2018) in press ; 1 - 1
by Mohamed A. Farag, Riham Salah El Din and Sherifa Fahmy
Abstract

In the published paper affiliations for coauthors Riham S. El Din & and Sherifa Fahmy is given a wrong number 2 and to be thus affiliated to Center for Regulatory and Environmental Analytical Metabolomics, Department of Chemistry, University of Louisville 2320 S. Brook St., Louisville and should be 1 to Pharmacognosy Department, College of Pharmacy, Kasr El-Aini St, Cairo University, Egypt. T he authors apologize for this error and their affiliation number changed as 1

DOI
http://doi.org/10.25135/rnp.88.18.07.325
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Magnoliaceae
Available online: July 18, 2018
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Original Article

8) Isomeric Phenolic Glycosides from from Populus tomentosa

Rec. Nat. Prod. (2018) in press ; 1 - 7
by Liang Xu, Haiping Liu, Yucui Ma, Cui Wu, Ruiqi Li and Zhimao Chao
Abstract

A new phenolic glycoside named salicyltomenside (1), with five similar compounds, salicyltremuloidin (2), tremuloidin (3), isograndidentatin A (4), siebolside B (5), and salicin (6) were isolated from Populus tomentosa Carr. Additionally, salicyltremuloidin (2) was obtained from the plant for the first time. Structure of the new compound was determined using spectroscopic methods including ESI-MS and 1D- and 2D-NMR. Others were elucidated through 1H and 13C NMR spectra and comparison with literature data. Notably, salicyltomenside (1) and salicyltremuloidin (2) were structural isomers.

DOI
http://doi.org/10.25135/rnp.82.18.03.257
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Populus tomentosa Carr. salicyltomenside salicyltremuloidin phenolic glycosides NMR.
Available online: July 13, 2018
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Short Report

9) Litsine A : A New A porphine A lkaloid from the Root Barks of Litsea glutinosa

Rec. Nat. Prod. (2018) in press ; 1 - 5
by Yan Jin, Younan Wu, Yiying Li, Caiyun Zhang, Wanying Sun Lin Dongand Xiaopo Zhang
Abstract

 A new aporphine alkaloid, named Litsine A, along with two known ones were isolated from the root barks of Litsea glutinosa . The new structure was determined by various spectroscopic techniques including 1D ( 1 H-, 13 C-NMR) , 2D-NMR (HMBC, HSQC, COSY and R OESY) and high resolution electrospray ionization mass spectrometry (HR-ESI-MS) , where as the structures of the known compounds were elucidated through 1D-NMR, mass spectrometric analysis and compared with the reported data in literature. The effects of these compounds on glucose uptake in C2C12 myotubes were evaluated at the concentration of 10μM. The three compounds exhibited potent activity , with 1 as the best one, in increasing glucose uptake.

DOI
http://doi.org/10.25135/rnp.87.18.04.276
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Litsea glutinosa Litsine A aporphine alkaloid s glucose uptake activities.
Available online: July 13, 2018
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Short Report

10) Chemical Composition, Antibacterial and Antioxidant Activities of Essential oil from Leonurus pseudomacranthus Kitag

Rec. Nat. Prod. (2018) in press ; 1 - 5
by Ling-Jing Mao , Xiao-Yu Xie, Yang Gao , Peng-Xiang Lai and Qing-lin Ma
Abstract

This study investigated the chemical composition and in vitro antibacterial and antioxidant activities of the essential oil obtained by hydrodistillation from the aerial parts ofLeonurus pseudomacranthus Kitag for the first time. The chemical composition was studied by GC-FID and GC-MS. Forty-nine compounds accounting for 91.1% of the essential oil were identified. The major components were s clareol (34.8%), β- c aryophyllene (7.1%), p recocene (I) (6.3%) and α- m uurolene (5.3%). The antibacterial activity of the essential oil was assessed by the disc diffusion and microdilution methods. The essential oil showed excellent antibacterial activity against Bacillus subtilis and Staphylococcus aureus with MIC values of 0.039 mg/mL and 0.156 mg/mL, respectively. Moreover, the antioxidant potential was evaluated by DPPH, ABTS and FRAP assays. The essential oil gave IC 50 values of 1.5 13 mg/mL, 0.1 52 mg/mL in DPPH and ABTS methods, and a Trolox equivalent concentration of 33.63 μmol Trolox × g -1 in FRAP method. The results indicated that the essential oil could be regarded as a promising product for pharmaceutical and food industry after more detailed study.

DOI
http://doi.org/10.25135/rnp.83.18.03.098
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Leonurus pseudomacranthus Kitag essential oil antibacterial activity antioxidant activity.
Available online: July 05, 2018
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Original Article

11) Phytochemical Changes in Aerial Parts of Hypericum perforatum at Different Harvest Stages

Rec. Nat. Prod. (2018) in press ; 1 - 9
by Ping Sun, Tianlan Kang, Hua Xing, Zhen Zhang, Delong Yang, Jinlin Zhang, Paul W. Paré and Mengfei Li
Abstract

Hypericum perforatum is a perennial herb that produces anti- depression metabolite hypericin (Hyp) as well as flavonoids and poly phenols . In order to find the optimization of harvest stage to increase bioactive compounds production , the levels of Hyp , flavonoids and poly phenols as well as antioxidant capacity were evaluated by the assays of high performance liquid chromatography (HPLC), NaNO 2-AlCl 3-NaOH and Folin-Ciocalteu as well as 1, 1-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP), respectively. The results showed that there was no difference in antioxidant capacity of extracts from aerial parts between floral budding stage (FBS) and blooming stage (BS) , while significant decrease was observed at fruit set stage (FSS) compared to BS (P < 0.05). On a per plant basis, biomass and the levels of Hyp , flavonoids and poly phenols in aerial parts decreased during harvest stages , even if bioactive compounds contents i n flower reache d the highest at BS on a dry weight basis . These findings indicated that the optimization of harvest stage of H. perforatum should be at FBS.

DOI
http://doi.org/10.25135/rnp.77.18.04.267
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Hypericum perforatum harvest stage hypericin flavonoids poly phenols antioxidant capacity .
Available online: June 08, 2018
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Original Article

12) Chemical Constituents and Anti-influenza Viral Activity of the Leaves of Vietnamese Plant Elaeocarpus tonkinensis

Rec. Nat. Prod. (2018) in press ; 1 - 10
by Nga Thu Dao, Yejin Jang, Meehyein Kim, Hung Huy Nguyen, Duong Quang Pham, Quang Le Dang, Minh Van Nguyen, Bong-Sik Yun, Quan Minh Pham, Jin-Cheol Kim and Vu Dinh Hoang
Abstract

Various Elaeocarpus species including Elaeocarpus tonkinensis have been important medicinal plants that used in traditional medication system and mainly used to cure nervous system-related disorders. However, their antiviral potential has not been reported yet. During the screening of medicinal plant extracts with the antiviral activity against influenza viruses, we found that E. tonkinensis extract has strong antiviral activity. Through organic solvent partition and repeated column chromatography using SiO 2, C-18 and Sephadex LH-20, a total of nine compounds were purified from the methanol extract of E. tonkinensis. Their chemical structures were determined by NMR and MS spectral data to be trolliamide (1), gallic acid (2), urolithin M-5 (3), hydroquinone (4), 2,4-dihydroxybenzoic acid (5), 3,5-dihydroxy-4-methoxybenzoic acid (6), corilagin (7), chebulagic acid (8), and shikimic acid (9). Their antiviral activity against influenza virus strains A/Puerto Rico/8/34 (H1N1; PR8), A/Hong Kong/8/68 (H3N2; HK) and B/Lee/40 (Lee) was examined on the basis of cytopathic effect (CPE) assay. Among them, compounds 2, 3, 4, 7, and 8 significantly inhibited viral replication in a dose-dependent manner with EC 50 values ranging from 7.8 to 59.6 μg/mL against influenza A and/or B viruses with selectivity indices above 5.0. This study suggests that the botanical materials of E. tonkinensis could be promising inhibitors of influenza A andB viruses and applied to the development of a novel herbal medicine.

DOI
http://doi.org/10.25135/rnp.76.18.04.266
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Influenza virus Elaeocarpus tonkinensi antiviral activity infectious disease
Available online: June 06, 2018
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Short Report

13) Chemical Composition and Antihypertensive Effect of Phoenix roebelenii Using Angiotensin Converting Enzyme inhibition invitro and in vivo

Rec. Nat. Prod. (2018) in press ; 1 - 6
by Karina S. Schumacker, Andrews Marques Nascimento, Anna Paula Rampazzo , Dominik Lenz, Helber Barcellos da Costa, Wanderson Romão, Rodrigo Scherer, Tadeu Uggere Andrade and Denise Coutinho Endringer
Abstract

This study aimed to evaluate in vivo anti-hypertensive effect of Phoenix roebelenii. To access the chemical composition, the EtOH extract and CH2Cl2 fraction of P. roebelenii were analyzed using electrospray ionization (ESI) source combined with the Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) technique. The ACE inhibitory effect was evaluated in vivo by Ang I administration. The antihypertensive assay was performed in Spontaneously Hypertensive Rats (SHR) and Wistar rats that were treated with enalapril (10 mg/kg), CH2Cl2 fraction (80 mg/kg; twice a day) or vehicle for 30 days. ACE activity in vivo was measured by colorimetric assay. ESI(-)FT-ICR mass spectrum for EtOH extract identified the presence of rutin, quercitrin, kaempferol-7-O-glucoside and kaempferol-3-O-rutenoside, and in the CH2Cl2 fraction, paradol, gingerol, ursolic/betulinic acid and maslinic/corosolic acid. CH2Cl2 fraction exhibited anti-hypertensive effect in vivo by reducing blood pressure in the SHR models. It may be concluded that the presence pungent vanilloids compounds in CH2Cl2 fraction contributed to the ACE inhibition in vitro and in vivo and that action could be the mechanism of the anti-hypertensive effect, known for its medicinal value

DOI
http://doi.org/10.25135/rnp.75.18.02.238
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Keywords:Phoenix roebelenii phoenix palm. angiotensin converting enzyme gingerol.
Available online: June 02, 2018
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Original Article

14) Isolation and Antimicrobial Activity of Rutin and Its Derivatives from Ruta chalepensis (Rutaceae) Growing in Iraq

Rec. Nat. Prod. (2018) in press ; 1 - 7
by Shaymaa Al-Majmaie, Lutfun Nahar, George P. Sharples, Khazal Wadi and Satyajit D. Sarker
Abstract

Rutin (1), rutin 3'-methyl ether (2), and a new flavonol glycoside, 6-hydroxy-rutin 3',7-dimethyl ether (3), were isolated from the methanol extract of the fruits of Ruta chalepensis , collected from Diyala, Iraq. Their structures were elucidated by spectroscopic analyses, including 1D-, 2D-NMR and HR ESI MS. Antimicrobial activity of compounds 1-3 was tested against four Gram +ve and Gram –ve bacterial strains, and the only fungal strain, Candida albicans, using the 96-well based resazurin microtitre assay.

DOI
http://doi.org/10.25135/rnp.74.18.03.250
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Ruta chalepensis Rutaceae resazurin assay antimicrobial activity rutin 6-hydroxy-rutin 3‵ 7-dimethyl ether
Available online: June 01, 2018
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Original Article

15) Chemical Characterization and Antioxidant Activity of Mountain Pine (Pinus mugo Turra, Pinaceae) from Republic of Macedonia

Rec. Nat. Prod. (2018) in press ; 1 - 14
by Marija Karapandzova, Gjoshe Stefkov, Ivana Cvetkovikj Karanfilova, Tatjana Kadifkova Panovska, Jasmina Petreska Stanoeva, Marina Stefova and Svetlana Kulevanova
Abstract

Chemical composition and antioxidant activity of essential oils and methanol extracts of needles of Pinus mugo native to R. Macedonia were investigated. Plant material was collected on Karadzica Mountain during two seasons. Essential oil was obtained by steam distillation and was analyzed by GC/FID/MS. 8 major constituents were identified: D 3 -carene, a -pinene, limonene+ b -phellandrene, germacrene D, trans-caryophyllene, d -cadinene, bicyclogermacrene and a -cadinol. Identification of flavonoids and phenolic compounds in methanol extracts was made by LC-DAD/ESI-MS n. 14 compounds were identified (2 phenolic acids, 2 procyanidins, 7 flavonol glycosides and 3 flavonol acylated glycosides). Total phenolic content (TPC) and total flavonoid content (TFC) were measured by Folin-Ciocalteau and aluminum chloride assay, respectively. TPC and TFC values ranged from 11.41-12.17 mgGAE/g and from 4.65-6.03 mgCE/g for dried plant material, respectively. Evaluation of antioxidant activity was made using DPPH and TBARS test. IC 50 values for the methanol extract ranged from 11.58-16.01 mg/mL for DPPH and from 9.41-15.76 mg/mL for TBARS assay. IC 50 values for the essential oil ranged from 2.51-4.26 mg/mL and 2.59-4.14 mg/mL for DPPH and TBARS assay, respectively. The needles from Macedonian P. mugo could be considered as a potential plant source for isolation of essential oil as well as flavonoids and other phenolics as they exhibit promising antioxidant activity. Additionally, f urther investigation and evaluation of chemical composition as well as biological activity should be done in order to provide more data for their possible medicinal, pharmaceutical and commercial utilization.

DOI
http://doi.org/10.25135/rnp.73.18.02.233
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Essential oil flavonoids GC/FID/MS LC/DAD/ESI-MS n methanol extract Pinus mugo
Available online: May 17, 2018
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Original Article

16) HPLC Fingerprint of Important Phenolic Compounds in Some Salvia L. Species from Iran

Rec. Nat. Prod. (2018) in press ; 1 - 13
by Marzieh Fotovvat, Tayebeh Radjabian, and Azra Saboora
Abstract

Salvia L. is a large genus of the Lamiaceae family with high medicinal value. Pharmaceutical properties of Salvia species are mainly due to their secondary metabolites, especially phenolic compounds. This study was focused on identification and determination of five bioactive phenolic compounds (rosmarinic acid, carnosic acid, caffeic acid, salvianolic acids A and B) in the 41 populations from 27 wild Salvia species of Iran using a simple and reliable HPLC-UV method. The principal component analysis (PCA) technique was used to study differentiation among species according to their phenolic compound profiles. Significant intra- and inter-specific variations were observed in the distribution patterns and contents of phenolic compounds in the studied Salvia species. As a result of this study, it was found that leaves had greater amounts of phenolic compounds as compared to the roots. The highest content of rosmarinic acid (41.53±0.88 mg/g DW) and salvianolic acid A (8.10±0.35 mg/g DW) were found in the leaves of S. verticillata. The leaves of S. syriaca and S. sharifii were rich in salvianolic acid B (54.47±2.00 mg/g DW) and carnosic acid (34.05±1.18 mg/g DW), respectively. The PCA results revealed chemical variations in the Salvia species collected from different regions and could fully distinguish between them based on the phenolic compounds concentrations. The present study demonstrated that apart from S. officinalis, some wild species such as S. verticillata, S. hypoleuca, S. leriifolia and S. virgata can be introduced as potent natural sources for medicinal and industrial purposes.

DOI
http://doi.org/10.25135/rnp.72.18.02.228
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Salvia species rosmarinic acid salvianolic acid A salvianolic acid B carnosic acid HPLC
Available online: May 15, 2018
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Original Article

17) Antinociceptive Activity of Essential Oils from Wild Growing and Micropropagated Plants of Renealmia alpinia (Rottb.) Maas

Rec. Nat. Prod. (2018) in press ; 1 - 8
by Isabel Gómez-Betancur, Dora Benjumea, Juan E. Gómez, Natalia Mejía and Juan F. León
Abstract

Renealmia alpinia is a medicinal plant that has been cultivated and used for the treatment of headaches, abdominal pain, as a febrifuge and t o treat snake bites in southeast Colombia and in the Caribbean islands. Moreover, its edible fruits are commonly used as spice in Mexico. The R. alpinia leave-extracts have shown enzymatic inhibition of Bothrops atrox and B othrops asper snake poison. The extracts also showed effects as a peripheral analgesic agent. This report investigates the analgesic activity of the essential oils obtained by hydrodistillation from wild and in vitro propagation samples of R. alpinia as well as the chemical profile using GC-MS. The essential oils isolated from R. alpinia evaluated on concentrations of 50, 75 and 100 mg/ k g showed significant antinociceptive effects in the test of acetic-acid-induced abdominal writhing, compared to the control animals. The essential oil of the wild flora leaves showed the highest percentage of pain inhibition by 200 mg/kg , concluding that t he essential oil of R. alpinia presents antinociceptive activity in an in vivo model. These results help us to understand the traditional use of R . a lpinia to treat pain and make it a potential source for the development of phyto pharmaceutics.

DOI
http://doi.org/10.25135/rnp.70.18.01.084
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Renealmia alpinia essential oil an tinociceptive micropropagation
Available online: April 30, 2018
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Original Article

18) The Chemical Composition and Biological Activity of the Essential Oil from the Underground Parts of Ferula tadshikorum (Apiaceae)

Rec. Nat. Prod. (2018) in press ; 1 - 6
by Farukh S. Sharopov, Payrav D. Khalifaev, Prabodh Satyal, Yanfang Sun, Abduahad Safomuddin, Safol Musozoda, Michael Wink and William N. Setzer
Abstract

The underground parts of Ferula  tadshikorum M. Pimenwere collected from the southern part of Tajikistan. The essential oils were obtained by hydrodistillation and analyzed by GLC-FID and GLC-MS. A total of 26 compounds were identified representing 94.4 % of total oil composition. The essential oil was dominated by the sulfur-containing compounds (Z)-1-propenyl sec-butyl disulfide (37. 3%), (E)-1-propenyl sec-butyl disulfide (29.9%), (E)-1-propenyl 1-(methylthio)propyl disulfide (16.8%), and propyl sec-butyl disulfide (4.8%). The antioxidant, antimicrobial and cytotoxic activities of the essential oil was evaluated. To our best knowledge, this is the first report concerning the chemical composition and biological activity of the essential oil obtained from the underground parts of F.  tadshikorum.

DOI
http://doi.org/10.25135/rnp.65.18.02.089
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Ferula tadshikorum essential oil composition disulfides
Available online: April 01, 2018
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Original Article

19) Biological Activities of Various Extracts from Salvia cassia Sam. ex Rech.f. and Chemical Composition of Its Most Active Extract

Rec. Nat. Prod. (2018) in press ; 1 - 13
by Burcu Sen Utsukarci, Bahar Gurda, Merve Bilgin, Dilek Satana, Betul Demirci, Nur Tan and Afife Mat
Abstract

This study was conducted on the antibacterial, antimycobacterial and antifungal activities of various extracts (petroleum ether (PE), chloroform (CHCl 3) and ethanol (EtOH) extracts; infusion and decoction) and essential oil from the aerial parts of S. cassia Sam. ex Rech.f. and the chemical composition of the most active samples. The antibacterial activity was determined against Bacillus subtilis, Staphylococcus aureus, methicillin resistant Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis, Klebsiella pneumoniae, Proteus mirabilis and Escherichia coli. The antimycobacterial activity was analyzed against five different Mycobacterium tuberculosis and the antifungal activity was compared with two dermotophytes and three Candida species. The composition of EO was analyzed by GC-FID and GC-MS. The EO had a very good activity against B. subtilis (0.3 µL/mL) and good activity against S. aureus, methicillin resistant S. aureus (MRSA) and E. faecalis (0.6 µL/mL). All extracts were active in the antimycobacterial and antifungal tests (MICs=50-400 µg/mL). The EO was the most active samples against all Mycobacterium strains, dermatophytes and Candida species (MICs=0.1-6.25 µL/mL). Because of the high activity of EO, its composition was analyzed, and the major compound was found caryophyllene oxide (22.3%), which was also found the major components of the essential oils from other antimicrobial plants such as Salvia trichoclada Benth.

DOI
http://doi.org/10.25135/rnp.68.18.02.090
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Salvia cassia antimicrobial antimycobacterial antifungal essential oil caryophyllene oxide
Available online: April 01, 2018
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Short Report

20) Investigation of Insect Repellent Activity of Cyclocolorenone Obtained from the Red Alga Laurencia intricata

Rec. Nat. Prod. (2018) in press ; 1 - 4
by Takahiro Ishii, Yuto Shinjo, Miyu Miyagi, Hiroshi Matsuura, Tsuyoshi Abe, Norio Kikuchi and Minoru Suzuki
Abstract

Three known secondary metabolites were isolated from the organic extract of Laurencia intricata. Their structures were identified by NMR and MS experiments and comparison with the literature data. An aromadendrane sesquiterpene, (+)-cyclocolorenone showed strong repellent activity against the maize weevil Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) adults. It had the same activity as natural insecticides, pyrethrins, with a ED 50 value of 2.0 m g/cm 2. This is the first report of insect repellent activity of (+)-cyclocolorenone. The results suggest that the red alga Laurencia is a promising source of bioactive compounds which can be used as potential biocontrol agents for stored-product insects.

DOI
http://doi.org/10.25135/rnp.66.18.02.221
(DOI number will be activated after the manuscript has been available in an issue.)
Keywords
Cyclocolorenone insect repellent activity Sitophilus zeamais Laurencia intricata
Available online: April 01, 2018
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© ACG Publications. All rights reserved.