JOURNAL 129


Journal of Chemical Metrology
VOLUME & ISSUE
Year: 2018 Issue: 1 January-June
PAGES
p.17 - 25
STATISTICS
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AUTHORS
    Ahmet C Gören, Gözde Şahin, İsmail Gümilcineli and Burcu Binici
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


The 25-OH Vitamin D2 and 25-OH Vitamin D3 measurement method was validated using a Zivak Multitasker Fully Automated Sample Preparation and Injection System coupled to a MS detector. The current method incorporates all sample preparations, SPE clean up procedures, LC and MS facilities into an all in one system. This system can report patient data within 6 minutes including the SPE and Sample preparation and mass spectrometry analysis time. The correlation coefficient of the developed method was greater than 0.99 for measurands, 25-OH-vitamin Ds, in the calibration range. The measurement uncertainty of the measurements were evaluated and found as 7.7 % and 7.6 % for 25-OH vitamin D2 and 25-OH-vitamin D3, respectively.

KEYWORDS
  • 25-OH Vitamin D2
  • 25-OH Vitamin D3
  • MS/MS
  • ZİVAK multitasker
  • method validation
  • uncertainty

SUPPORTING INFORMATION


Supporting Information
Download File 2-13-JCM-1802-070-SI.pdf (566.34 KB)