Journal of Chemical Metrology
Year: 2018 Issue: 2 July-December
p.121 - 127
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  • Sema Çarıkçı
  • Turgut Kılıç
  • Züleyha Özer
  • Tuncay Dirmenci
  • Turan Arabaci
  • Ahmet C Gören


One of the most important phytochemicals found in plants is phenolics. Phenolic compounds have antioxidant properties  due to -OH group attached to aromatic ring. Phenolic substances give the antioxidant properties to the plants. So, identifying and developing analytical methods for the determination of phenolics are very important. In this study, quantities of some phenolics; caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin in the directly methanol (M1), chloroform (C), acetone (Ac) and and methanol (M2) extratcs obteined from Origanum laevigatum Boiss. from Turkey were investigated via  liquid chromatography and tandem mass spectrometry (LC-MS/MS). Curcumin was used as an internal standard. In the extracts, caffeic acid, chlorogenic acid, rosmarinic acid and gallic acid determined as the most abundant phenolic acids for M1, C, Ac, and M2, respectively. The relative standard deviations (RSD) for caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol, and vanillin were found to be 8.04, 5.21, 5.45, 3.73, 5.44, 4.85, 5.47 and 6.57 % respectively. For the investigated analytes,  the correlation coefficient was found in the range of  0.9803 to 0.9981. Furthermore, antioxidant properties of the extracts were determined based on 2,2-diphenyl-1- picrylhydrazyl (DPPH), β-carotene linoleic acid and cupric (Cu2+) ion reducing power assay (CUPRAC). Acetone (Ac) and methanol (M2) extracts were showed high activity in all test assays

  • Origanum
  • phenolics
  • LC-MS/MS
  • validation
  • antioxidant activity
  • rosmarinic acid


Supporting Information
Download File 21.JCM.1812.1115-SI.pdf (579.69 KB)