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Sesamin and 4-hydroxy-sesamin from Cinnamomum camphora: extraction, purification and anti-inflammatory effects on the BV2 microglia by LPS-induced

Original Article

JOURNAL 3437

Journal of Chemical Metrology
VOLUME & ISSUE
Year: 2025 Issue: 1 January-June
PAGES
p.44 - 62
DOI ADDRESS
http://doi.org/10.25135/jcm.111.2502.3437
STATISTICS
Viewed 783 times.
AUTHORS
  • Qi Sun
  • Fen Liu
  • Jiyu Zhang
  • Qiang Song
  • Mengfei Xu
  • Mingfeng Chen
  • Xiaoqin Zhang
  • Lin Ni
PDF OF ARTICLE

GRAPHICAL ABSTRACT

ABSTRACT

This study optimized the extraction of sesamin and 4-hydroxy-sesamin from Cinnamomum camphora residue, yielding 0.3804 % under conditions of 86 ℃, 43 min, and a 1:8.39 solid-liquid ratio. The purified compounds showed 67.86 % and 78.87 % recovery rates via recrystallization. In LPS-induced BV-2 microglia, 4-hydroxy-sesamol (10-40 μmol/L) activated autophagy, reduced iNOS, COX-2, IL-6, IL-1β, TNF-α mRNA expression, and demonstrated neuroprotection without cytotoxicity, suggesting its potential in mitigating neuroinflammation.

 KEYWORDS
  • Cinnamomum camphor
  • response surface methodology
  • silica gel column chromatography
  • anti-inflammatory activity

SUPPORTING INFORMATION

Supporting Information
Download File 111-JCM-2502-3437-SI.pdf (276.06 KB)