JOURNAL 21


Records of Natural Products
VOLUME & ISSUE
Year: 2008 Issue: 4 October-December
PAGES
p.107 - 115
STATISTICS
Viewed 1783 times.
AUTHORS
    Himanshu Misra, Bhupendra K. Mehta and Dharam C. Jain
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GRAPHICAL ABSTRACT


ABSTRACT


A simple, precise and accurate high-performance thin-layer chromatographic method has been established for quantitative determination of quinine. Conditions were also optimized for best possible extraction of quinine via varying concentrations of diethyl amine in different solvents (n-hexane, chloroform, ethyl acetate and methanol) for maximum recovery of quinine. Methanol modified with 20 % DEA found to be best for highest possible recovery of target analyte quinine. Chromatographic separation of quinine was performed on silica gel 60 F 254 HPTLC plates with ethyl acetate : diethyl amine in the proportion 88 : 12 (v/v), as mobile phase. The determination was carried out using the densitometric absorbance mode at 236 nm. Quinine response was found to be linear over the range 4–24 μg spot −1. The HPTLC method was evaluated in terms of specificity, precision, reproducibility, LOD – LOQ and robustness. Beside these parameters, number of theoretical plates and flow constant were also included as a part of validation.

KEYWORDS
  • Cinchona spp.
  • Rubiaceae
  • bark
  • quinine
  • HPTLC determination.