JOURNAL 2985


Journal of Chemical Metrology
VOLUME & ISSUE
Year: 2023 Issue: 2 July-December
PAGES
p.249 - 256
STATISTICS
Viewed 602 times.
AUTHORS
  • Burhan Ceylan
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


Amount of α-escin and β-escin in cosmetic cream formulations were determined by simple reverse phase HPLC method by using the C18 column at 228 nm. The mobile phase is composed of acetonitrile-water (60:40) at pH 2.4. The method validation study was carried out based on the ICH recommendations and linear regression equations were determined as y=1548.3x+340.7 and y=1521.3x-91.9, for the analytes, respectively. While LOD values of the method were determined for α-escin and β-escin as 0.003 µg/mL and 0.027 µg/mL,  LOQ values were determined as 0.01 µg/mL and 0.08 0.01 µg/mL, respectively

KEYWORDS
  • Horse chesnut
  • α-escin
  • β-escin
  • HPLC-UV
  • Validation