Journal of Chemical Metrology
A scientific open access journal in the field of analytical chemistry and accreditation
Journal of Chemical MetrologyA scientific open access journal in the field of analytical chemistry and accreditation
Development of a quick reverse phase liquid chromatographic method with photodiode-array detection for quantitative determination of chlorthalidone, metoprolol succinate and telmisartan in tablet formulation
A quick and simple reversed-phase High-Performance Liquid Chromatographic method (RP-HPLC) with isocratic elution has been developed and validated for the concurrent quantitative determination of chlorthalidone, metoprolol succinate and telmisartan in the bulk mixture and tablet dosage form. The chromatographic separation was performed using Inertsil Octadecyl-Silica (ODS- C18) Column (150mm×4.6mm, 5μm) stationary phase. The separation and elution was carried out using a mobile phase mixture of phosphate buffer (50 mM, pH 2.5) and acetonitrile at a ratio of 40:60 v/v and 0.7 mL/min flow rate. Chlorthalidone, metoprolol succinate and telmisartan were eluted at 2.96±0.5 min, 4.31±0.5 min and 5.94±0.5 min respectively. The response was recorded using a photodiode array (PDA) detector at 225 nm wavelength. The selected method was linear in the range of 3.21-18.72 µg/mL, 6.25-37.50 µg/mL and 10-60 µg/mL while percentage recovery was in the range from 99.15 to 99.93%, 99.15 to 99.42% and 100.03 to 100.08% for chlorthalidone, metoprolol succinate and telmisartan respectively. The method was found to be sensitive, selective, linear and reproducible and the results obtained suggest the suitability of the developed method for routine analysis of the formulations containing the combination of these drugs.DOI http://doi.org/10.25135/jcm.6012106.2098 Keywords Chlorthalidone high-performance liquid chromatography metoprolol Succinate telmisartan validation Available online: August 24, 2021 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.
Isolation of amygdalin epimer at high diastereomeric purity and its structural characterization by spectroscopic and Q-TOF LC-MS methods
Amygdalin, a cyanogenic glycoside mostly found in the bitter almond kernel, plays an important role in conventional and modern medicine applications. The high diastereomeric purity amygdalin was isolated with chromatographic purification methods and recrystallization. Amygdalin and neoamygdalin were separated chromatographically using the porous graphitic carbon column. Identification and confirmation of each molecule was performed with the ESI Quadrupole Time of Flight system coupled with a reversed phase LC.
DOI http://doi.org/10.25135/jcm.60.2105.2076 Keywords Amygdalin neoamygdalin cyanogenic glycoside LC-MS Q-TOF chromatography Available online: August 18, 2021 DETAILS DOWNLOAD PDF © ACG Publications. All rights reserved.