Journal of Chemical Metrology

A scientific open access journal in the field of analytical chemistry and accreditation
Editor-in-Chief: Mustafa Özyürek
Book Review and Review Article Editor: John Warren

LATEST ARTICLES

Original Article

Development of a quick reverse phase liquid chromatographic method with photodiode-array detection for quantitative determination of chlorthalidone, metoprolol succinate and telmisartan in tablet formulation

J. Chem. Metrol. (2021) in press ; 1 - 9
by Avani Chokshi , Rohit Prajapati , Pritesh Desai and Mehul Patel

A quick and simple reversed-phase High-Performance Liquid Chromatographic method (RP-HPLC) with isocratic elution has been developed and validated for the concurrent quantitative determination of chlorthalidone, metoprolol succinate and telmisartan in the bulk mixture and tablet dosage form. The chromatographic separation was performed using Inertsil Octadecyl-Silica (ODS- C18) Column (150mm×4.6mm, 5μm) stationary phase. The separation and elution was carried out using a mobile phase mixture of phosphate buffer (50 mM, pH 2.5) and acetonitrile at a ratio of 40:60 v/v and 0.7 mL/min flow rate. Chlorthalidone, metoprolol succinate and telmisartan were eluted at 2.96±0.5 min, 4.31±0.5 min and 5.94±0.5 min respectively. The response was recorded using a photodiode array (PDA) detector at 225 nm wavelength. The selected method was linear in the range of 3.21-18.72 µg/mL, 6.25-37.50 µg/mL and 10-60 µg/mL while percentage recovery was in the range from 99.15 to 99.93%, 99.15 to 99.42% and 100.03 to 100.08% for chlorthalidone, metoprolol succinate and telmisartan respectively. The method was found to be sensitive, selective, linear and reproducible and the results obtained suggest the suitability of the developed method for routine analysis of the formulations containing the combination of these drugs.

DOI
http://doi.org/10.25135/jcm.6012106.2098
Keywords
Chlorthalidone high-performance liquid chromatography metoprolol Succinate telmisartan validation
Available online: August 24, 2021
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© ACG Publications. All rights reserved.
Original Article

Isolation of amygdalin epimer at high diastereomeric purity and its structural characterization by spectroscopic and Q-TOF LC-MS methods

J. Chem. Metrol. (2021) in press ; 1 - 14
by Pshtiwan Raheem Sabır , İrfan Çapan , Ayhan İbrahim Aysal and Süleyman Servi

Amygdalin, a cyanogenic glycoside mostly found in the bitter almond kernel, plays an important role in conventional and modern medicine applications. The high diastereomeric purity amygdalin was isolated with chromatographic purification methods and recrystallization. Amygdalin and neoamygdalin were separated chromatographically using the porous graphitic carbon column. Identification and confirmation of each molecule was performed with the ESI Quadrupole Time of Flight system coupled with a reversed phase LC.

 

DOI
http://doi.org/10.25135/jcm.60.2105.2076
Keywords
Amygdalin neoamygdalin cyanogenic glycoside LC-MS Q-TOF chromatography
Available online: August 18, 2021
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© ACG Publications. All rights reserved.