Journal of Chemical Metrology

Articles In Press

Original Article

1) Trace element analysis in corn by cloud point extraction

J. Chem. Metrol. (2024) in press ; 1 - 11
by Mustafa S. Dundar , Sedef Arpaözü , Celal Caner , Huseyin Altundag , Özcan Güleç and Mustafa Arslan

In this study, Lead (Pb), Copper (Cu), and Tin (Sn) elements determined in corn samples were analyzed from three different regions of Sakarya province. A cloud point extraction (CPE) method was used for the metal determination study and analysis by inductively coupled plasma-optical emission spectroscopy (ICP-OES). In this study, optimization processes were performed in cloud point extraction 2-(4-sulphonylamidebenzo)hydrazide-1-dithiocarbamate salt as ligand and Triton X-114 as surfactant. Three different corn samples, soil samples, and certified reference materials (GBW10011 and GBW10012) were analyzed. Recovery values in corn samples were 95.5-97.1% for Cu, 101.8-105.2% for Pb, and 94.1-97.3% for Sn; Recovery values for soil samples were 99.3-104.3% for Cu, 93.6-103.3% for Pb and 99.5-101.6% for Sn detected among them.

DOI
http://doi.org/10.25135/jcm.109.2403.3180
Keywords
Corn trace element 2-(4-sulphonylamidebenzo)hydrazide-1-dithiocarbamate salt ICP-OES cloud point extraction (CPE).
Available online: May 11, 2024
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Original Article

2) Development of liquid chromatographic (LC) method for simultaneous estimation of novel anti diabetic drug Evogliptin and Metformin

J. Chem. Metrol. (2024) in press ; 1 - 10
by Deepanti Gajjar and Dimalkumar A Shah

Evogliptin and Metformin are antidiabetic medications. Evogliptin works by increasing the release of insulin from the pancreas and decreasing the hormones that raise blood sugar levels. This reduces the fasting and post-meal sugar levels. Metformin works by lowering glucose production in the liver, delaying the absorption of sugar (glucose) from the intestines, and increasing the body's sensitivity to insulin. Accurate and precise high performance liquid chromatographic method has been developed for the estimation of Metformin and Evogliptin. Agilent C18 Column (250mm x 4.6mm, 5µm particle size) was used as stationary phase and methanol: 0.11% acetic acid in water (31: 69 % V/V) was used as mobile phase. The method was linear in the concentration range 50-250 µg/mL and 0.5 to 2.5 µg/mL of Metformin and Evogliptin respectively with a correlation coefficient of 0.999. The proposed method was validated with respect to linearity, accuracy, precision, and robustness as per International Council for Harmonisation of Technical Requirements of Pharmaceuticals for Human Use ICH Q2 (R2) guideline. The method was successfully applied for the analysis of Metformin and Evogliptin

DOI
http://doi.org/10.25135/jcm.108.2312.2989
Keywords
Metformin evogliptin design of experiment validation accuracy robustness
Available online: March 28, 2024
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Original Article

3) Novel analytical method development and validation for simultaneous estimation of curcumin, ascorbic acid and salicylic acid in bulk and its pharmaceutical formulation by RP-HPLC

J. Chem. Metrol. (2024) in press ; 1 - 9
by Manisha Jadav , Vandana Patel and Lalit Jha

A precise and specific reverse phase high-performance liquid chromatographic method has been developed and validated to quantify curcumin, ascorbic acid, and salicylic acid in both bulk and hydrogel. Utilizing a Hypersil BDS C18 column and an isocratic mode, the mobile phase comprised of a mixture of 0.1% orthophosphoric acid and acetonitrile (50:50 v/v). The calibration range spanned concentrations of 100 - 300 µg/mL for curcumin, 50 - 150 µg/mL for ascorbic acid and 50 - 150 µg/mL for salicylic acid. The specificity of the proposed method for estimating these compounds was established through chromatographic peak purity analysis. The limit of detection and the limit of quantification were found to be 18.54 µg/mL and 56.20 µg/mL for curcumin, 10.05 µg/mL and 30.46 µg/mL for ascorbic acid, and 11.39 µg/mL and 34.51 µg/mL for salicylic acid respectively. The accuracy of the method was demonstrated by recovering curcumin, ascorbic acid, and salicylic acid from the hydrogel formulation with a recovery rate exceeding 98%. This indicates the capability of the method to accurately estimate active pharmaceutical ingredients in hydrogel dosage form without interference from excipients. Validation results support the potential applicability of the proposed method for the quantitative estimation of these three drugs in hydrogel.

DOI
http://doi.org/10.25135/jcm.107.2310.2936
Keywords
High Performance liquid chromatography Hydrogel Method Development Curcumin Ascorbic acid Salicylic acid
Available online: March 15, 2024
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Original Article

4) RP-HPLC method combined with ultrafiltration for simultaneous analysis of Melphalan and Topotecan in human plasma samples

J. Chem. Metrol. (2024) in press ; 1 - 11
by Ozan Kaplan , Nergiz Yılmaz , Büşra Uçar , Merve Özge Algedik , İrem Koç , Hayyam Kıratlı and Mustafa Çelebier

Pharmaceutical analysis still attracts the attention of researchers, and pharmaceutical analysis methods specific to the purpose of the application are needed in routine applications. In this study, the RP-HPLC method was developed for the simultaneous analysis of two different cancer drugs from human blood plasma due to simultaneous use. Melphalan and topotecan are licensed drugs that have been used for a long time. In routine practice, the simultaneous use of these two drugs is limited. However, studies have found that two active substances were used together in high-dose chemotherapy applications. This situation encourages the development of methods for the simultaneous analysis of both active substances in spiked human blood plasma samples. In this study, melphalan and topotecan were analyzed by RP-HPLC in 17 minutes, including post-run, with a gradient elution program using a Superco 5 µm C18 100 Å LC Column (100 x 4.6 mm) when the flow rate was 1.0 mL min-1. The method was linear in the 1.0- 20.0 µg/mL range for both active substances. The detection wavelength was 261 nm. The accuracy and precision of the validated analytical method were demonstrated through intraday and interday studies. The analyte was freed from the remaining plasma components due to filtering the supernatant (after precipitation of the plasma proteins with methanol) with an ultrafilter (having 10 kDa pores). The simple methodology used in this study can be easily adapted to any pre-clinical or clinical application where analysis of melphalan and topotecan in plasma is required.

DOI
http://doi.org/10.25135/jcm.105.2311.2962
Keywords
Melphalan Topotecan RP-HPLC pharmaceutical analysis ultrafiltration validation
Available online: March 09, 2024
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Original Article

5) Development of an RP-HPLC method to evaluate the basic characteristics of talazoparib-loaded PLGA nanoparticles

J. Chem. Metrol. (2024) in press ; 1 - 9
by Beril Tas Topcu , Ozan Kaplan , Sibel Bozdağ Pehlivan , Mustafa Çelebier and Levent Öner

The use of poly (ADP-ribose) polymerase (PARP) inhibitors for cancer treatment has been reported previously. Talazoparib is a PARP inhibitor, and its solubility problems encouraged us to prepare talazoparib-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles for use in brain cancer models. To determine the encapsulation efficiency and release profile, a reversed-phase high-pressure liquid chromatography (RP-HPLC) method was developed and validated. A Shiseido 5 µm C18 100 Å column (250 × 4.6 mm) was used with a flow rate of 1.0 mL/min. Isocratic elution was performed using an acetonitrile:phosphate buffer (100 mm, pH 6.25) (35:65 v/v) mixture. The injection volume was 5 μL and UV detection was performed at 227 nm. The method was linear within the range from 0.1 to 12.5 µg/mL. The encapsulation efficiency and release profile of the prepared formulation were analyzed using the developed RP-HPLC method, and it was found that the encapsulation efficiency was 65.17% ± 0.50 and the release of the talazoparib was around 40% within 5 h and remained stable for 25 h. The RP-HPLC method developed in the present study can be adapted for further applications to determine talazoparib in its commercial formulations and proposed encapsulated drug delivery systems.

DOI
http://doi.org/10.25135/jcm.2310.2937
Keywords
Talazoparib RP-HPLC nanoparticles drug delivery method development
Available online: February 24, 2024
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Original Article

6) Simultaneous estimation of dapagliflozin and linagliptin using reverse phase-HPLC with photo diode array (PDA)

J. Chem. Metrol. (2024) in press ; 1 - 11
by Anchal Shukla , Usmangani K. Chhalotiya , Dimal Shah , Jinal Tandel , Hetaben Kachhiya and Mital Parmar

Dapagliflozin inhibits selective sodium–glucose co-transporter-2 and Linagliptin competitively and reversibly inhibits dipeptidyl peptidase-4 in fixed dose Combination (1:1) is used in the treatment of Type 2 Diabetes Mellitus. For estimation Dapagliflozin and Linagliptin in bulk and Tablet formulation, an accurate and precise method using RP-HPLC was developed and validated. In the method being discussed here was optimized using Hypersil C18 (250 × 4.6 mm, 5 µm) column as Stationary Phase, Mobile Phase being used is Acetonitrile: Water (90:10) adjusting pH 3 using Ammonium Acetate. The Flow Rate was adjusted to 1ml/min. Both Dapagliflozin and Linagliptin (1:1) sample was detected at analytical wavelength of 244nm using Photo diode array detector. The Linearity Range was between Concentration of 0.1µg/ml to 20µg/ml with correlation Coefficient of 0.995 and 0.999 for Dapagliflozin and Linagiliptin Respectively.

DOI
http://doi.org/10.25135/jcm.104.2401.3020
Keywords
Diabetes Mellitus dapagliflozin linagliptin RP-HPLC method validation
Available online: February 14, 2024
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Original Article

7) Development and application of RP–HPLC method for estimation and evaluation of stability of brinzolamide in ophthalmic formulation

J. Chem. Metrol. (2024) in press ; 1 - 9
by Mehul Patel , Ritu Dwivedi , Umang Shah and Hetaben Kachhiya

This study outlines the development, optimization, and validation of a robust reverse-phase high-performance liquid chromatography (RP-HPLC) technique for the precise quantification of Brinzolamide in ophthalmic products, aligning International Council for Harmonization (ICH) guidelines. The method employed a Phenomenex (C18) (250×4.6mm) column with 5μm particle size as the stationary phase, a 1 mL/min flow rate for the mobile phase (composed of acetonitrile: water 35:65 v/v, pH adjusted to 3 with orthophosphoric acid), and detection at 254 nm. Under these conditions, Brinzolamide displayed Rt of 4.9 minutes. The validation process, following ICH standards, exhibited excellent linearity within the 5–30 μg/mL concentration range, with a limit of detection at 0.22 μg/mL and a limit of quantification at 0.67 μg/mL. Recovery rates from ophthalmic formulations fell between 98.3%-101.08%, indicating high accuracy. Accelerated stability assessments conducted over three months revealed content retention between 98.2%-100.9%, affirming the product's stability. Additionally, Brinzolamide withstood various stress conditions without interference in quantification, as the degradation products had distinct retention times from the pure drug, offering excellent resolution. In conclusion, this RP-HPLC method is suited for routine quality control analysis of Brinzolamide in commercial ophthalmic preparations, due to its specificity, accuracy, precision, and sensitivity, aligning perfectly with ICH guidelines.

DOI
http://doi.org/10.25135/jcm.2311.2949
Keywords
Brinzolamide RP-HPLC method accelerated stability study force degradation degradation products
Available online: January 11, 2024
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