JOURNAL 3562
Journal of Chemical Metrology
Year: 2025 Issue: 1 January-June
p.94 - 102
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GRAPHICAL ABSTRACT
ABSTRACT
Triticum Vulgare extract is a natural wound healer widely used in pharmaceutical production cream formulations due to its ability to accelerate tissue repair. In this study, method validation and measurement uncertainty budget of a developed ion chromatography method to determine the amount of active oligosaccharide in the finished product formulation were reported. A Thermo Dionex Carbopac PA1 (4 mm x 250 mm) column connected to a Thermo Dionex Carbopac PA1 Guard (4 mm x 50 mm) guard column was used as the analytical column in the High-Performance Anion Exchange Chromatography with Emphasized Amperometric Detector method. A total run time of the method was applied as 85 minutes and 95% to 5% gradient of Mobile Phase A (Sodium Hydroxide) and Mobile Phase B (Sodium Acetate Trihydrate-Sodium Hydroxide) solutions were used with 0.5 mL/min flow rate The developed method was validated in terms of specificity, precision, linearity and range, accuracy and recovery parameters according to the ICH Q2 (R2) guideline. The linear regression equation of the method was determined as y=6204.20x-737.0873 (r:0.999), while % recovery was obtained as 103.4± 3.6.
KEYWORDS- Triticum vulgare L.
- ion chromatography
- method validation
- cream
- extract
